Enhanced Sensory Coding in Mouse Vibrissal and Visual Cortex through TRPA1

Transient receptor potential ankyrin 1 (TRPA1) is a non-selective cation channel, broadly expressed throughout the body. Despite its expression in the mammalian brain, little is known about the contribution of TRPA1 to cortical function. Here, we characterize how TRPA1 affects sensory information processing in two cortical areas in mice: the primary vibrissal (whisker) somatosensory cortex (vS1) and the primary visual cortex (V1). In vS1, local activation of TRPA1 by allyl isothiocyanate (AITC) increases the ongoing activity of neurons and their evoked response to vibrissal stimulation, producing a positive gain modulation. The gain modulation is reversed by TRPA1 inhibitor HC-030031 and is absent in TRPA1 knockout mice. Similarly, in V1, TRPA1 activation increases the gain of direction and orientation selectivity. Linear decoding of V1 population activity confirms faster and more reliable encoding of visual signals under TRPA1 activation. Overall, our findings reveal a physiological role for TRPA1 in enhancing sensory signals in the mammalian cortex.