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An Integrative Analysis of Transcriptome, Proteome and Hormones Reveals Key Differentially Expressed Genes and
Danlong Jing1,2, Weiwei Chen1,2, Ruoqian Hu2
1Key Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Beibei, Chongqing 400715, China.
International Journal of Molecular Sciences
|July 24, 2020
Summary
This study reveals key genes and proteins regulating loquat flower development, highlighting hormone signaling and carbohydrate metabolism pathways essential for floral transition and organ identity.
Area of Science:
- Plant Biology
- Molecular Biology
- Biochemistry
Background:
- Flower development is crucial for woody perennials, particularly fruit trees, influencing reproductive success.
- Understanding the molecular mechanisms of flower development is key to improving fruit tree cultivation.
Purpose of the Study:
- To investigate the key candidate genes, proteins, and hormones involved in loquat (Eriobotrya japonica) flower development.
- To identify molecular players and pathways regulating flower bud differentiation, elongation, and anthesis.
Main Methods:
- Transcriptomic analysis to identify differentially expressed genes (DEGs).
- Proteomic analysis to identify differentially accumulated proteins (DAPs).
- Hormone concentration measurements (GA3, zeatin, ABA).
Main Results:
- DEGs were enriched in hormone signal transduction (auxin, GA, cytokinin, ethylene, ABA) and starch/sucrose metabolism.
- Key floral integrator genes (FT, SOC1) and meristem identity genes (SPL, LFY, AP1, AP2) were upregulated at the FBD stage.
- Floral organ identity genes (AG, AP3, PI) were upregulated during FBE and FA stages.
- Transcription factors (bHLH, NAC, MYB, ERF, C2H2) showed differential expression.
- Starch and sucrose metabolism was significantly enriched in both transcriptomic and proteomic data.
- Hormone levels showed distinct patterns, with high GA3 and zeatin before FA, and sustained high ABA at FA.
Conclusions:
- Identified candidate genes, proteins, and hormone pathways critical for loquat flower development.
- Provides valuable molecular resources for further research into fruit tree reproductive biology and breeding.
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