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Long non-coding RNA PHACTR2-AS1 promotes tongue squamous cell carcinoma metastasis by regulating Snail

Fenqian Yuan1, Zhiguo Miao2, Wen Chen3

  • 1Department of Head and Neck Surgery, Jiangxi Cancer Hospital, 519 East Beijing Road, Nanchang 330029, Jiangxi, China.

Journal of Biochemistry
|July 24, 2020
PubMed

Insights

Long non-coding RNA PHACTR2-AS1 promotes tongue squamous cell carcinoma metastasis by sponging miR-137 and upregulating Snail. This finding offers a potential therapeutic target for metastatic TSCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are key regulators in cancer biology.
  • The role of PHACTR2-AS1 in tongue squamous cell carcinoma (TSCC) remains largely unexplored.

Purpose of the Study:

  • To investigate the biological function and clinical significance of PHACTR2-AS1 in TSCC.
  • To elucidate the underlying molecular mechanism of PHACTR2-AS1 in TSCC progression.

Main Methods:

  • Quantitative real-time PCR to assess PHACTR2-AS1 expression in TSCC tissues and cell lines.
  • Cell migration, invasion, and lung metastasis assays in vivo and in vitro.
  • MiRNA-RNA immunoprecipitation and Western blot assays to explore the molecular mechanism.

Main Results:

  • PHACTR2-AS1 was significantly upregulated in TSCC tissues and correlated with poor prognostic factors.
  • PHACTR2-AS1 depletion inhibited TSCC cell migration, invasion, and lung metastasis.
  • PHACTR2-AS1 acts as a molecular sponge for miR-137, increasing Snail expression and promoting epithelial-mesenchymal transition.

Conclusions:

  • PHACTR2-AS1 plays a critical role in promoting TSCC cell metastasis.
  • PHACTR2-AS1 represents a potential therapeutic target for patients with metastatic TSCC.
Keywords:
PHACTR2-AS1epithelial–mesenchymal transitionmetastasisprognosistongue squamous cell carcinoma

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