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Dark Blood Magnetic Resonance Imaging for Visualizing Inflamed Intracranial Arteries01:14

Dark Blood Magnetic Resonance Imaging for Visualizing Inflamed Intracranial Arteries

Start with a human patient with cerebral vasculitis, which causes inflammation and increased permeability of blood vessels in the brain.Place the patient's head inside the radiofrequency, or RF, coil of a magnetic resonance imaging, or MRI, machine.Initiate magnetic resonance angiography, or MRA, which uses RF pulses to image blood flow.Protons in flowing blood generate strong signals, making blood vessels appear bright, while weak signals from stationary tissues result in a dark...
153
Cell-Based Drug Screening for Inhibitors of Autophagy Related 4B Cysteine Peptidase09:51

Cell-Based Drug Screening for Inhibitors of Autophagy Related 4B Cysteine Peptidase

Here, we describe a detailed protocol for the use of a luciferase-based reporter assay in a semi-automated, high-throughput screening...
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A Novel Approach to Monitoring Graft Neovascularization in the Human Gingiva07:51

A Novel Approach to Monitoring Graft Neovascularization in the Human Gingiva

This study introduces a protocol for measuring microcirculation in human oral mucosa by laser speckle contrast imaging. The monitoring of wound healing after vestibuloplasty combined with a xenogenic collagen graft is presented on a clinical...
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Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva

The goal of the protocol is to build an inflammatory human gingiva model in vitro. This tissue model co-cultivates three types of human cells, HaCaT keratinocytes, gingival fibroblasts, and THP-1 macrophages, under three-dimensional conditions. This model can be applied to investigating periodontal diseases, such as gingivitis and...
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Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry09:14

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

This protocol details the important steps required for the bioconjugation of a cysteine containing protein to a maleimide, including reagent purification, reaction conditions, bioconjugate purification and bioconjugate...
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Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis11:28

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

The mechanisms that govern the interstitial motility of CD4 effector T cells at sites of inflammation are relatively unknown. We present a non-invasive approach to visualize and manipulate in vitro-primed CD4 T cells in the inflamed ear dermis, allowing for study of the dynamic behavior of these cells in...
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