Related Experiment Video

Updated: Dec 14, 2025

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
08:48

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Published on: November 22, 2019

7.9K

High-peak-power 918-nm laser light source based two-photon spinning-disk microscopy for green fluorophores

Kohei Otomo1, Ai Goto2, Yumi Yamanaka2

  • 1Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, 5-1, Higashiyama, Myodaiji, Okazaki, 444-8787, Japan; National Institute for Physiological Sciences, 5-1, Higashiyama, Myodaiji, Okazaki, 444-8787, Japan; Graduate School of Advanced Studies Sciences (SOKENDAI), 5-1, Higashiyama, Myodaiji, Okazaki, 444-8787, Japan; Research Institute for Electronic Science, Hokkaido University, Kita 20 Nishi 10, Kita, Sapporo, 001-0020, Japan; Graduate School of Information Science and Technology, Hokkaido University, Kita 14 Nishi 9, Kita, Sapporo, 001-0014, Japan.

Biochemical and Biophysical Research Communications
|July 25, 2020
PubMed

Keywords:
Ca(2+) imagingMulti-point laser scanningNeodymium laserSpinning-disk confocal microscopyTwo-photon microscopy

More Related Videos

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures12:58

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures

Published on: March 26, 2019

15.4K
Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy10:19

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Published on: January 21, 2019

6.8K

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.3K