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LncRNA CASC15 functions as an oncogene by sponging miR-130b-3p in bladder cancer

X Yu1, Z-L Wang, C-L Han

  • 1Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.

European Review for Medical and Pharmacological Sciences
|July 25, 2020
PubMed

Insights

This article on LncRNA CASC15 in bladder cancer has been withdrawn due to suspected research misconduct. The publisher retracted the study after authors failed to verify data originality.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) play critical roles in cancer development.
  • Aberrant expression of lncRNAs is implicated in various human malignancies, including bladder cancer.
  • Specific lncRNAs can act as competing endogenous RNAs (ceRNAs) to regulate gene expression through microRNA (miRNA) sponging.

Purpose of the Study:

  • To investigate the role of LncRNA CASC15 in bladder cancer.
  • To explore the interaction between LncRNA CASC15 and miR-130b-3p.
  • To determine if LncRNA CASC15 functions as an oncogene in bladder cancer.

Main Methods:

  • The study aimed to analyze the expression of LncRNA CASC15 and miR-130b-3p in bladder cancer tissues and cell lines.
  • Mechanisms of action were investigated through in vitro experiments, including cell proliferation, migration, and invasion assays.
  • Bioinformatic analyses and luciferase reporter assays were employed to confirm the sponging activity of LncRNA CASC15 on miR-130b-3p.

Main Results:

  • LncRNA CASC15 was found to be upregulated in bladder cancer.
  • Overexpression of LncRNA CASC15 promoted bladder cancer cell proliferation, migration, and invasion.
  • LncRNA CASC15 was identified to directly bind to miR-130b-3p, acting as a sponge.

Conclusions:

  • LncRNA CASC15 acts as an oncogene in bladder cancer by sponging miR-130b-3p.
  • LncRNA CASC15 may serve as a potential therapeutic target for bladder cancer treatment.

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