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Long non-coding RNA OIP5-AS1 plays an oncogenic role in ovarian cancer through targeting miR-324-3p/NFIB axis

Q-Y Liu1, X-X Jiang, H-N Tian

  • 1Department of Gynaecology, Rizhao People's Hospital, Rizhao, China. hekesongkeng1598@163.com.

European Review for Medical and Pharmacological Sciences
|July 25, 2020
PubMed
Abstract

Insights

Long non-coding RNA OIP5-AS1 accelerates ovarian cancer (OC) progression by sponging miR-324-3p and upregulating NFIB. This suggests OIP5-AS1 is a potential therapeutic target for OC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) play roles in ovarian cancer (OC) progression.
  • The specific functions of lncRNA-OIP5-AS1 in OC remain largely unexplored.

Purpose of the Study:

  • To investigate the molecular mechanisms of OIP5-AS1 in ovarian cancer.
  • To elucidate the role of OIP5-AS1 in OC progression and its potential as a therapeutic target.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to assess expression levels of OIP5-AS1, miR-324-3p, and NFIB.
  • Cell viability, invasion, and migration assays (CCK-8, Transwell) to evaluate OC cell behavior.
  • Western blot analysis for NFIB protein levels and Dual-Luciferase reporter assay for molecular interactions.

Main Results:

  • OIP5-AS1 and NFIB were upregulated, while miR-324-3p was downregulated in OC tissues and cell lines.
  • OIP5-AS1 knockdown and miR-324-3p overexpression inhibited OC cell viability, invasion, and migration.
  • OIP5-AS1 was found to inhibit miR-324-3p, which directly targets NFIB, and NFIB silencing suppressed OC cell progression.

Conclusions:

  • OIP5-AS1 promotes OC progression by sponging miR-324-3p and upregulating NFIB.
  • OIP5-AS1 represents a potential therapeutic target for ovarian cancer treatment.

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