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Complex Microbiota in Laboratory Rodents: Management Considerations.

Craig L Franklin1,2,3, Aaron C Ericsson1,2,3

  • 1Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri.

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Summary
This summary is machine-generated.

The gut microbiota (GM) significantly impacts health and disease. Understanding and accounting for its complex variations in research is crucial for reproducibility and future therapeutic applications in humans and animals.

Keywords:
Clinical genomicsfeces bankinggut microbiotamicrobiota transferreproducibilitytranslatability

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Area of Science:

  • Microbiology
  • Genomics
  • Animal Models

Background:

  • The human body harbors diverse bacterial communities, known as microbiota, across nearly all organ systems.
  • Historically, characterizing these microbiota relied on culturing methods, which failed to identify the vast majority of microbes, especially those in anaerobic environments like the gut.
  • Advancements in next-generation sequencing have enabled comprehensive microbiota characterization without cultivation, leading to an explosion of data and new interpretative challenges.

Purpose of the Study:

  • To discuss the importance of including complex microbiota in experimental design for improved model reproducibility.
  • To explore strategies for exploiting the natural variation within rodent microbiota research colonies.
  • To highlight the potential of harnessing microbial communities for human and animal health benefits.

Main Methods:

  • Review of current understanding of microbiota composition and its impact on host phenotype.
  • Discussion of next-generation sequencing technologies for microbiota analysis.
  • Exploration of experimental design considerations for incorporating microbiota variability.

Main Results:

  • Microbiota, particularly the gut microbiota (GM), plays a critical role in host health and disease susceptibility.
  • Significant variation in microbiota composition exists even within healthy individuals and animal models.
  • Subtle microbiota differences can influence animal model phenotypes, potentially translating to human disease susceptibility.

Conclusions:

  • Integrating microbiota considerations into experimental design is essential for enhancing reproducibility in research.
  • The inherent variability in microbiota presents opportunities for novel research and therapeutic strategies.
  • Harnessing the genetic potential of host-associated microbes may offer future benefits for human and animal health.