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Combinatory Treatment with miR-7-5p and Drug-Loaded Cubosomes Effectively Impairs Cancer Cells

Ewa Gajda1, Marlena Godlewska1, Zenon Mariak2

  • 1Department of Biochemistry and Molecular Biology, Centre of Postgraduate Medical Education, Marymoncka 99/103, 01-813 Warsaw, Poland.

International Journal of Molecular Sciences
|July 26, 2020
PubMed
Abstract

Insights

This study shows that combining microRNA-7-5p (miR-7-5p) with chemotherapy in cubosomes enhances anti-tumor effects and overcomes multidrug resistance (MDR) in cancer cells.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cancer Research

Background:

  • Multidrug resistance (MDR) poses a significant challenge in cancer treatment.
  • Novel strategies are needed to sensitize drug-resistant tumor cells.
  • MicroRNA (miRNA) therapy and drug-loaded cubosomes are innovative approaches.

Purpose of the Study:

  • To investigate a novel approach combining miR-7-5p and chemotherapeutics within cubosomes.
  • To evaluate the efficacy of this combined therapy against various cancer cell lines.

Main Methods:

  • Development of cubosomes co-loaded with miR-7-5p and chemotherapeutics.
  • Analysis of cellular response using quantitative real-time PCR, MTS assay, flow cytometry, and spheroids formation assay.
  • Testing on glioma, papillary thyroid, and cervical carcinoma cell lines.

Main Results:

  • miR-7-5p significantly increased cancer cell sensitivity to chemotherapeutics.
  • Co-loaded cubosomes demonstrated a greater anti-tumor effect than free drugs.
  • Apoptosis induction was observed, with altered expression of ABC family MDR proteins.

Conclusions:

  • Co-administration of miR-7-5p with chemotherapeutics is a promising strategy.
  • This approach effectively reduces multidrug resistance.
  • The combination therapy induces apoptosis in cancer cells.
Keywords:
cancer cellscubosomesdoxorubicinglioblastomamiR-7-5pmultidrug resistancetemozolomide

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