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Updated: Dec 13, 2025
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Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain
Published on: August 28, 2012
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Amyloid precursor protein 770 is specifically expressed and released from platelets.
Saori Miura1, Akiomi Yoshihisa2, Tomofumi Misaka2
1Preparing Section for New Faculty of Medical Science, Fukushima Medical University, Fukushima, Japan.
The Journal of Biological Chemistry
|July 26, 2020
Summary
Soluble β-Amyloid precursor protein (APP) 770 (sAPP770) is a novel biomarker for platelet activation. Plasma sAPP770 levels correlate with platelet activation and decrease with antiplatelet therapy in coronary artery disease patients.
Area of Science:
- Cardiovascular Biology
- Hemostasis and Thrombosis
- Biomarker Discovery
Background:
- Platelets are crucial in hemostasis but also implicated in coronary artery disease (CAD) and cerebrovascular lesions.
- Antiplatelet therapy is recommended for CAD patients but increases bleeding risk.
- Accurate assessment of platelet activation and therapy response is needed.
Purpose of the Study:
- To evaluate soluble β-Amyloid precursor protein (APP) 770 (sAPP770) as a specific biomarker for platelet activation.
- To investigate the relationship between plasma sAPP770 levels and platelet activation status in patients with CAD.
Main Methods:
- Analysis of plasma sAPP770 levels in CAD patients and controls.
- Correlation analysis with soluble CD40 ligand (CD40L), a known platelet activation marker.
- Flow cytometry to assess APP770 expression in blood cells and sAPP770 release upon platelet stimulation.
Main Results:
- Plasma sAPP770 levels positively correlated with CD40L, a marker of platelet activation.
- APP770 expression was found predominantly in platelets, not other blood cells.
- Platelet stimulation with collagen or ADP rapidly released sAPP770.
- Patients on dual antiplatelet therapy had significantly lower plasma sAPP770 levels.
Conclusions:
- Plasma sAPP770 is a specific and promising biomarker for assessing platelet activation.
- sAPP770 levels reflect platelet activation status and response to antiplatelet therapy.
- This biomarker could aid in managing antiplatelet therapy in cardiovascular patients.
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