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Transfection of MDCK cells with influenza virus ribonucleoprotein complexes
1Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid.
Microbiologia (Madrid, Spain)
|December 1, 1988
Abstract:
Influenza virus ribonucleoprotein complexes isolated from MDCK-infected cells have been used to optimize transfection conditions of MDCK cells. Ribonucleoprotein complex-mediated infection was strictly dependent on pretreatment of the cell cultures, resistant to mild NP40 treatment and sensitive to RNase treatment. Under optimal conditions, up to 10(4) plaque forming units per microgram of viral RNA could be obtained.