Related Experiment Video

Updated: Dec 13, 2025

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
13:59

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Published on: August 12, 2018

8.5K

tBHP treatment as a model for cellular senescence and pollution-induced skin aging

Sophia Wedel1, Ines Martic1, Nina Hrapovic2

  • 1Institute for Biomedical Aging Research, University of Innsbruck, Austria; Center for Molecular Biosciences Innsbruck (CMBI), Innsbruck, Austria.

Mechanisms of Ageing and Development
|July 26, 2020
PubMed

Insights

Tert-butyl hydroperoxide (tBHP) induces cellular senescence and oxidative stress, mimicking extrinsic skin aging. This model reveals tissue damage, highlighting environmental pollution

Area of Science:

  • Dermatology
  • Cell Biology
  • Aging Research

Background:

  • Cellular senescence contributes to age-related diseases and skin aging.
  • Senescent cells in skin degrade extracellular matrix and alter tissue function.
  • Oxidative stress and reactive oxygen species are key drivers of senescence and skin aging.

Purpose of the Study:

  • To characterize tert-butyl hydroperoxide (tBHP)-induced senescence in human dermal fibroblasts.
  • To evaluate tBHP's effects on physiological skin models.
  • To establish tBHP as a model for studying extrinsic skin aging, particularly environmental pollution impacts.

Main Methods:

  • Induction of senescence in human dermal fibroblasts using tBHP.
  • Treatment of 3D reconstructed skin and ex vivo skin with tBHP.
  • Assessment of cellular and tissue damage, including epidermal thickness and collagen integrity.

Main Results:

  • tBHP effectively induced senescence and oxidative stress in fibroblasts.
  • tBHP treatment in 3D skin models and ex vivo skin led to chronic tissue damage.
  • Observed effects included reduced epidermal thickness and thinning of collagen fibers.

Conclusions:

  • tBHP serves as a valuable tool for modeling extrinsic skin aging in vitro.
  • The study highlights the detrimental effects of oxidative stress on skin structure and function.
  • This model can be utilized to investigate the impact of environmental pollutants on skin aging.
Keywords:
cigarette smokecollagen degradationepidermal thinningoxidative stress

More Related Videos

Induction and Validation of Cellular Senescence in Primary Human Cells08:18

Induction and Validation of Cellular Senescence in Primary Human Cells

Published on: June 20, 2018

17.7K
Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate DMBA-TPA04:12

Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate DMBA-TPA

Published on: December 19, 2019

15.1K

Related Concept Videos

Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
4.2K
Aging01:26

Aging

Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
490
The Effect of Aging on Tissues01:19

The Effect of Aging on Tissues

Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
3.1K
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
3.0K
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.9K