Development of a molecular K⁺ probe for colorimetric/fluorescent/photoacoustic detection of K

The potassium ion (K⁺) plays significant roles in many biological processes. To date, great efforts have been devoted to the development of K⁺ sensors for colorimetric, fluorescent, and photoacoustic detection of K⁺ separately. However, the development of molecular K⁺ probes for colorimetric detection of urinary K⁺, monitoring K⁺ fluxes in living cells by fluorescence imaging, and photoacoustic imaging of K⁺ dynamics in deep tissues still remains an open challenge. Herein, we report the first molecular K⁺ probe (NK2) for colorimetric, fluorescent, and photoacoustic detection of K⁺. NK2 is composed of 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) as the chromophore and phenylazacrown-6-lariat ether (ACLE) as the K⁺ recognition unit. Predominate features of NK2 include a short synthetic procedure, high K⁺ selectivity, large detection range (5-200 mM), and triple-channel detection manner. NK2 shows good response to K⁺ with obvious color changes, fluorescence enhancements (about threefold), and photoacoustic intensity changes. The existence of other metal ions (including Na⁺, Mg²⁺, Ca²⁺, Fe²⁺) and pH changes (6.5-9.0) have no obvious influence on K⁺ sensing of NK2. Portable test strips stained by NK2 can be used to qualitatively detect urinary K⁺ by color changes for self-diagnosis of diseases induced by high levels of K⁺. NK2 can be utilized to monitor K⁺ fluxes in living cells by fluorescent imaging. We also find its excellent performance in photoacoustic imaging of different K⁺ concentrations in the mouse ear. NK2 is the first molecular K⁺ probe for colorimetric, fluorescent, and photoacoustic detection of K⁺ in urine, in living cells, and in the mouse ear. The development of NK2 will broaden K⁺ probes' design and extend their applications to different fields. Graphical abstract.