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Updated: Dec 13, 2025

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
Published on: August 13, 2021
Analysis of metabolically labeled inositol phosphate messengers by NMR
Robert Puschmann1, Robert K Harmel1, Dorothea Fiedler1
1Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Berlin, Germany; Institut für Chemie, Humboldt-Universität zu Berlin, Berlin, Germany.
Abstract:
Inositol phosphates (InsPs) are an important group of eukaryotic messengers and mediate a wide range of processes. To elucidate the biological functions of these molecules, robust techniques to characterize inositol phosphate metabolism at the cellular level are highly sought after. This chapter provides a detailed protocol for the preparation of 13C-labeled myo-inositol, its use for metabolic labeling of mammalian and yeast cells, and the quantitative analysis of intracellular InsP pools from cell extracts using NMR spectroscopy.
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