Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA sequence patterns in precisely positioned nucleosomes.

E C Uberbacher1, J M Harp, G J Bunick

  • 1University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences, Biology.

Journal of Biomolecular Structure & Dynamics
|August 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Computational tools for protein modeling.

Current protein & peptide science·2002
Same author

Methylation of histone H3 by coactivator-associated arginine methyltransferase 1.

Biochemistry·2001
Same author

Asymmetries in the nucleosome core particle at 2.5 A resolution.

Acta crystallographica. Section D, Biological crystallography·2000
Same author

Sequence-structure specificity of a knowledge based energy function at the secondary structure level.

Bioinformatics (Oxford, England)·2000
Same author

Crystal structure and mechanism of a carbon-carbon bond hydrolase.

Structure (London, England : 1993)·1999
Same author

Macromolecular crystal annealing: evaluation of techniques and variables.

Acta crystallographica. Section D, Biological crystallography·1999
Same journal

Decrypting the allosteric network: multi-scale dynamics of SARS-CoV-2 main protease inhibition by <i>Moringa oleifera</i> flavonoids.

Journal of biomolecular structure & dynamics·2026
Same journal

Anti-mycobacterial activity of phytocompounds from <i>Ricinus communis</i> L. - an integrated <i>in-vitro</i> and <i>in-silico</i> approach.

Journal of biomolecular structure & dynamics·2026
Same journal

Binding studies of the X-ray characterized [SnMe<sub>2</sub>Cl<sub>2</sub>(Me<sub>2</sub>phen)] complex with human serum albumin: experimental and molecular docking approaches.

Journal of biomolecular structure & dynamics·2026
Same journal

Computational design and experimental validation of peptide inhibitors to disrupt urease enzyme maturation in pathogenic bacteria <i>Proteus mirabilis</i>.

Journal of biomolecular structure & dynamics·2026
Same journal

Wavelet-domain multiway spectral separation of free drug, DNA, and drug-DNA complex profiles for quantitative binding analysis based on fractional occupancy (<i>θ</i>).

Journal of biomolecular structure & dynamics·2026
Same journal

Gene expression and microsecond scale conformational dynamics suggest potential regulatory mechanisms for the expanded subtilase family of <i>T. rubrum</i>.

Journal of biomolecular structure & dynamics·2026
See all related articles

DNA sequence, including non-periodic information, significantly influences nucleosome positioning. Dinucleotide preferences reveal unique positional patterns, aiding in predicting nucleosome locations.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biophysics

Background:

  • Nucleosome positioning is crucial for DNA accessibility and gene regulation.
  • Both periodic and non-periodic DNA sequence features are implicated in nucleosome positioning.

Purpose of the Study:

  • To quantify the contribution of non-periodic DNA sequence information to nucleosome positioning.
  • To characterize the periodic sequence information influencing precisely positioned nucleosomes.

Main Methods:

  • Analysis of half-nucleosome DNA sequences from precisely positioned nucleosomes.
  • Positional probability assessment of each dinucleotide type from the nucleosome center.

Main Results:

  • Identified significant DNA sequence periodicities (6-7, 10, 21 base pairs) and non-periodic information.

Related Experiment Videos

  • Demonstrated unique positional preferences for each dinucleotide type (e.g., AA vs. TT).
  • Established that dinucleotide probabilities can predict nucleosome translational positions.

    • Conclusions:

    • Non-periodic DNA sequence information is vital for precise nucleosome positioning.
    • Dinucleotide composition and its positional context are key determinants of nucleosome placement.
    • This study provides a framework for predicting nucleosome positions based on sequence analysis.