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Subcellular Fractionation

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

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Updated: Dec 13, 2025

Fractionation of Lignocellulosic Biomass using the OrganoCat Process

Fractionation of Lignocellulosic Biomass using the OrganoCat Process

Published on: June 5, 2021

Lignin Fractionation in Segmented Continuous Flow.

Reza Ebrahimi Majdar^1,2, Claudia Crestini^3,4,5, Heiko Lange^4,6,5

¹Department of Chemical Sciences and Technologies, University of Rome 'Tor Vergata', Via della Ricerca Scientifica, 00133, Rome, Italy.

|July 28, 2020

Summary

This summary is machine-generated.

Segmented continuous flow fractionation (SCFF) offers precise control over lignin properties. This automated method efficiently separates lignin into valuable fractions with tunable molecular weights and physicochemical characteristics.

Keywords:

31P NMR spectroscopy fractionation gel permeation chromatography lignin segmented continuous flow

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Last Updated: Dec 13, 2025

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Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids

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Published on: August 10, 2016

Area of Science:

Biomass valorization and polymer chemistry.

Background:

Lignin, a complex biopolymer, presents challenges in selective fractionation for industrial applications.
Conventional methods often lack the precision and control needed for tailored lignin-derived materials.

Purpose of the Study:

To develop and evaluate a segmented continuous flow fractionation (SCFF) technique for precise lignin separation.
To explore the potential of SCFF in generating lignin fractions with distinct molecular weight and physicochemical properties.

Main Methods:

Lignin fractionation using SCFF with varied isocratic/gradient solvent systems, flow rates, temperatures, and pressures.
Remote control and automation capabilities of the SCFF process.
Analysis of fractionated lignins using gel permeation chromatography (GPC) and 31P NMR spectroscopy.

Main Results:

SCFF enabled superior control over fractionation parameters, yielding industrially relevant lignin fractions.
The process demonstrated successful scale-up in both linear and parallel modes.
SCFF produced fractions comparable to conventional batch methods, alongside novel fractions achievable with pressurized, overheated solvent systems.

Conclusions:

Segmented continuous flow fractionation is a versatile and controllable method for lignin valorization.
SCFF facilitates the production of tailored lignin fractions for diverse applications.
The technique offers potential for automated, scalable, and precise lignin processing.