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Updated: Dec 13, 2025

Proper Care and Cleaning of the Microscope
Published on: August 11, 2008
Efficacy of Cleaning Methods for Ophthalmic Microscopic Instruments: A Comparison Study
Abstract:
The combination of silicone oil and blood is difficult to remove from ophthalmic surgical instruments during cleaning and decontamination processes. We sought to establish the most efficient cleaning procedure for this type of contaminated instrument. We uniformly contaminated microscopic instruments made of titanium alloy and stainless steel with either blood alone or blood and silicone oil. We randomly assigned each instrument to one of four types of cleaning procedures that involved combinations of water, a multi-enzyme detergent, or an alkaline detergent. After completing the designated cleaning procedure, a sterile processing technician used an adenosine triphosphate cleaning verification test to evaluate the cleaning efficacy. When cleaning blood- and silicone oil-contaminated titanium-alloy and stainless-steel instruments, the alkaline detergent immersion followed by a multi-enzyme detergent ultrasonic cleaning yielded the highest cleaning effectiveness score (92.5%), which indicates this was the most effective of the four cleaning procedures that we tested.
Insights
The most effective method for cleaning ophthalmic surgical instruments contaminated with blood and silicone oil involves alkaline detergent immersion followed by multi-enzyme ultrasonic cleaning. This procedure achieved the highest cleaning effectiveness score in the study.
Area of Science:
- Ophthalmology
- Surgical Instrument Decontamination
- Biomedical Engineering
Background:
- Blood and silicone oil are challenging contaminants on ophthalmic surgical instruments.
- Effective cleaning is crucial for instrument reprocessing and patient safety.
Purpose of the Study:
- To determine the optimal cleaning procedure for ophthalmic surgical instruments contaminated with blood and silicone oil.
- To compare the efficacy of different cleaning agents and methods.
Main Methods:
- Microscopic instruments made of titanium alloy and stainless steel were contaminated with blood or blood and silicone oil.
- Instruments were subjected to one of four cleaning procedures involving water, multi-enzyme detergent, or alkaline detergent.
- Adenosine triphosphate (ATP) testing was used to verify cleaning efficacy.
Main Results:
- The combination of alkaline detergent immersion followed by multi-enzyme ultrasonic cleaning demonstrated the highest cleaning effectiveness.
- This optimized procedure achieved a 92.5% cleaning effectiveness score.
- The tested procedure was superior to other combinations of cleaning agents and methods.
Conclusions:
- Alkaline detergent immersion followed by multi-enzyme ultrasonic cleaning is the most effective method for decontaminating ophthalmic surgical instruments soiled with blood and silicone oil.
- This finding provides critical guidance for sterile processing departments to improve cleaning protocols.
- Optimized cleaning protocols enhance the safety and longevity of surgical instruments.
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