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Surface enhanced Raman scattering of bacteria using capped and uncapped silver nanoparticles.

P A Mosier-Boss¹, K C Sorensen², R D George²

¹GEC, 5101B Backlick Rd., Annandale, VA 22003, United States of America.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy

|July 28, 2020

Summary

Surface enhanced Raman scattering (SERS) using silver nanoparticles (Ag NPs) reveals differences in bacterial interactions. Capped Ag NPs bind cell membranes, while uncapped Ag NPs detect purine degradation metabolites.

Keywords:

Bacteria Borohydride Citrate Secretions Surface enhanced Raman scattering (SERS)

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Area of Science:

Biophysics
Microbiology
Nanotechnology

Background:

Surface-enhanced Raman scattering (SERS) is a powerful technique for analyzing biological samples.
Silver nanoparticles (Ag NPs) are commonly used as SERS substrates.
Bacterial cell surface structures can influence nanoparticle interactions.

Purpose of the Study:

To investigate the impact of silver nanoparticle (Ag NP) capping on SERS spectra of bacteria.
To understand how different Ag NP types interact with bacterial cell envelopes.
To correlate SERS spectral differences with specific bacterial components and metabolic activities.

Main Methods:

Generation of citrate-capped and borohydride-uncapped silver nanoparticles (Ag NPs).
Acquisition of SERS spectra from bacteria incubated with both types of Ag NPs.
Analysis of spectral differences to infer nanoparticle-bacteria interactions.

Main Results:

Citrate-capped Ag NPs interacted with bacterial cell membranes and periplasmic space, yielding spectra from cell envelope components and secretions.
Borohydride-uncapped Ag NPs could not penetrate the bacterial polysaccharide layer, resulting in spectra primarily from purine degradation metabolites.
Distinct SERS spectral profiles were observed, correlating with the different interaction mechanisms of capped versus uncapped Ag NPs.

Conclusions:

The surface chemistry of Ag NPs significantly dictates their interaction with bacterial cells.
SERS, utilizing tailored Ag NPs, can differentiate between surface and internal bacterial components.
This approach offers potential for targeted bacterial analysis and metabolic profiling.