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Updated: Dec 13, 2025

Growth of Mycobacterium tuberculosis Biofilms
Published on: February 15, 2012
Stability studies with tigecycline in bacterial growth medium and impact of stabilizing agents
Lisa F Amann1, Emilia Ruda Vicente1, Mareike Rathke1
1Department of Clinical Pharmacy, Institute of Pharmacy, University of Hamburg, Bundesstraße 45, DE-20146, Hamburg, Germany.
Purpose:
This study aimed to examine the degradation of tigecycline in Mueller Hinton broth (ca-MHB), as knowledge about bacterial susceptibility is key for therapeutic decisions.
Methods:
Antioxidative stabilizers were evaluated on tigecycline stability in a quantitative chromatography assay and tigecycline induced kill against Staphylococcus aureus (ATCC29213) was determined in time kill studies.
Results:
Ascorbic acid caused rapid degradation of tigecycline and resulted in loss of antibacterial activity. Tigecycline was stabilized in aged broth by 2% pyruvate and bacterial growth, and tigecycline killing was similar to fresh broth without supplementation, but independent of age.
Conclusion:
Our results underline the importance of using freshly prepared ca-MHB or the need for stabilizers for tigecycline susceptibility testing while using aged ca-MHB.
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