Circular RNA circRHOBTB3 inhibits ovarian cancer progression through PI3K/AKT signaling pathway

Related Experiment Video

Updated: Dec 13, 2025

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

3.0K

Yalan Sun1, Yanfang Liu1, He Chen1

  • 1Department of Obstetrics, Luoyang Central Hospital, Zhengzhou University, Luoyang, China.

Panminerva Medica
|July 29, 2020
PubMed
Abstract

Insights

Circular RNA RHOBTB3 (circRHOBTB3) suppresses ovarian cancer progression by inhibiting cell proliferation, metastasis, and glycolysis. It functions by inactivating the PI3K/AKT signaling pathway, offering a potential therapeutic target for ovarian cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Circular RNAs (circRNAs) are increasingly recognized as key regulators in human cancers.
  • Circular RNA RHOBTB3 (circRHOBTB3) is implicated in various cellular processes.

Purpose of the Study:

  • To investigate the functional role and molecular mechanisms of circRHOBTB3 in ovarian cancer.
  • To determine if circRHOBTB3 acts as a tumor suppressor in ovarian cancer.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) and Western blot were used to assess gene and protein expression.
  • Cell counting kit-8 (CCK-8), transwell assays, and cell fractionation identified functional impacts and localization.
  • In vivo tumor models and PI3K/AKT pathway modulators were employed to validate findings.

Main Results:

  • circRHOBTB3 was significantly downregulated in ovarian cancer tissues and cells.
  • Overexpression of circRHOBTB3 suppressed ovarian cancer cell proliferation, migration, invasion, and glycolysis.
  • circRHOBTB3 inactivation of the PI3K/AKT signaling pathway was confirmed.

Conclusions:

  • circRHOBTB3 acts as a tumor suppressor in ovarian cancer.
  • Inhibition of the PI3K/AKT pathway by circRHOBTB3 underlies its anti-tumorigenic effects.
  • circRHOBTB3 represents a potential therapeutic target for ovarian cancer treatment.

More Related Videos

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

2.1K
Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

7.1K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.0K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.4K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.1K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.5K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.4K