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Complex assembly, crystallization and preliminary X-ray crystallographic analysis of duck MHC class I complexed with

Zixin Liu1, Xiaoli Xie1, Zhuolin Li1

  • 1Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing, China.

Research in Veterinary Science
|July 30, 2020
PubMed

The CTL immune response mediated by MHC I plays an important role in duck anti-TMUV infection. This study reports the expression, purification and crystallization of a complex of duck MHC class I molecules Anpl-UAA*SD, duck β2-microglobulin (Anpl-β2m) and the polypeptide LRKRQLTVL (LRK9) derived from Tembusu virus (TMUV) NS3. The crystal diffraction resolution is 1.50 Å and belongs to the P62 space group, and the unit cell parameters are a = 82.468, b = 82.468, c = 112.507. The Matthew's constant is calculated to be 2.32 Å3 Da -1, and an asymmetric unit contains a complex molecule with a solvent content of 47%. The research lays the foundation for the structure of immune molecules about duck anti-TMUV research.

Keywords:
Complex assemblyCrystallizationDuckMHC class IPeptideTMUV

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