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Fabrication and Characterization of Ceftizoxime-Loaded Pectin Nanocarriers
Pawan Kumar1, Vinod Kumar2, Ravinder Kumar3
1Department of Materials Science and Nanotechnology, Deenbandhu Chhotu Ram University of Science and Technology, Murthal 131039, India.
Insights
Pectin nanocarriers successfully delivered ceftizoxime, a third-generation cephalosporin antibiotic, showing sustained release and potent antimicrobial activity against various bacteria. This novel nanoformulation offers a promising approach for treating bacterial infections.
Area of Science:
- Pharmaceutical Sciences
- Materials Science
- Nanotechnology
Background:
- Ceftizoxime is a third-generation cephalosporin antibiotic effective against bacterial infections.
- Pectin, a biopolymer, offers biocompatibility and tunable properties for drug delivery applications.
- Developing effective nanocarriers is crucial for enhancing antibiotic efficacy and stability.
Purpose of the Study:
- To synthesize and optimize ceftizoxime-loaded pectin nanocarriers (CPN).
- To characterize the physicochemical properties and in vitro drug release of CPN.
- To evaluate the antimicrobial activity of CPN against specific bacterial strains.
Main Methods:
- Ceftizoxime-loaded pectin nanocarriers (CPN) were synthesized using the solvent displacement method.
- Response surface methodology with Design-Expert software was employed for nanoformulation optimization.
- In vitro characterization included particle size, morphology (TEM), FTIR, and drug release studies via dialysis.
- Antimicrobial activity was assessed against Bacillus cereus, Bacillus polymyxa, Enterobacter aerogenes, and Pseudomonas aeruginosa.
Main Results:
- TEM revealed irregular shaped nanoparticles ranging from 29-110 nm.
- In vitro studies demonstrated sustained drug release of ceftizoxime from pectin nanocarriers over 24 hours.
- CPN exhibited significant antimicrobial activity against all tested bacterial pathogens.
Conclusions:
- Pectin serves as a suitable nanocarrier for ceftizoxime, enhancing its delivery.
- The developed CPN formulation exhibits sustained drug release and effective antimicrobial properties.
- This pectin-based nanoformulation holds potential for improved treatment of bacterial infections.
Abstract:
Ceftizoxime (C13H12N5NaO5S2)is a parenteral, third-generationcephalosporin antibiotic used to treat bacterial infections including ear, nose, and throat infections. In this work, pectin has been used as a nanocarrier for ceftizoxime due to its high biocompatibility and non-toxicity with tunable surface properties. Ceftizoxime-loaded pectin nanocarriers (CPN) were successfully synthesized by the solvent displacement method. Optimization of nanoformulation was done by response surface methodology using Design-Expert software. The optimized formulation examined various in-vitro characterizations such as particle size, morphology, and FTIR studies. TEM results revealed irregular shape nanoparticles within the range of 29-110 nm. The in-vitro drug release using the dialysis method was performed after 24h where nanoformulation showed sustained drug release. Drug-loaded nanoparticles revealed good antimicrobial activity against Bacillus cereus, Bacilluspolymyxa, Enterobacteraerogenes, andPseudomonasaeruginosa.
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