Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

8.3K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
8.3K
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

21.2K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
21.2K
What is Genetic Engineering?00:49

What is Genetic Engineering?

79.2K
Overview
79.2K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.4K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.4K
Plant Tissue Culture02:57

Plant Tissue Culture

39.8K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
39.8K
CRISPR01:59

CRISPR

56.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
56.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Virus-mediated, heritable gene editing in groundcherry (<i>Physalis grisea</i>).

Frontiers in plant science·2026
Same author

At the breaking point: developmental and molecular insights into <i>Physalis grisea</i> fruit abscission.

Frontiers in plant science·2026
Same author

A neofunctionalized flowering antagonist created an evolutionary contingency that channeled Solanaceae adaptation.

bioRxiv : the preprint server for biology·2026
Same author

Solanum pan-genetics reveals paralogues as contingencies in crop engineering.

Nature·2025
Same author

PlantGENE: Advancing plant transformation through community engagement.

The Plant journal : for cell and molecular biology·2025
Same author

Convergent evolution of plant prickles by repeated gene co-option over deep time.

Science (New York, N.Y.)·2024

Related Experiment Video

Updated: Dec 13, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.0K

Applying gene editing to tailor precise genetic modifications in plants.

Joyce Van Eck1

  • 1Boyce Thompson Institute, Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, New York, USA.

The Journal of Biological Chemistry
|July 30, 2020
PubMed
Summary

CRISPR-Cas gene editing revolutionizes plant genome modification, enabling precise DNA alterations for crop improvement. This technology facilitates site-specific changes, enhancing gene function studies and crop traits.

Keywords:
CRISPR/CasCas proteinsDNA-free gene editingbase editinggene knockoutgene silencinggene transfermultiplex editingplantplant gene editingprime editing

More Related Videos

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants
08:48

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

10.9K
Author Spotlight: Advancing Gene Editing in Bamboo Leaves for Sustainable Plastic Alternatives
06:57

Author Spotlight: Advancing Gene Editing in Bamboo Leaves for Sustainable Plastic Alternatives

Published on: August 18, 2023

2.1K

Related Experiment Videos

Last Updated: Dec 13, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.0K
Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants
08:48

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

10.9K
Author Spotlight: Advancing Gene Editing in Bamboo Leaves for Sustainable Plastic Alternatives
06:57

Author Spotlight: Advancing Gene Editing in Bamboo Leaves for Sustainable Plastic Alternatives

Published on: August 18, 2023

2.1K

Area of Science:

  • Genetics and Genomics
  • Plant Biotechnology
  • Molecular Biology

Background:

  • Advancements in genome sequencing and gene editing technologies have accelerated site-specific alterations in plant genomes.
  • Gene editing provides a foundation for gene function studies, metabolic engineering, and crop trait improvement.

Purpose of the Study:

  • To review the evolution and application of gene editing technologies, particularly CRISPR-Cas, in plant science.
  • To summarize current approaches for precise genome modification in plants, from single-base changes to fragment insertion.
  • To discuss strategies for modulating gene expression and the prerequisites and challenges of CRISPR-Cas-mediated editing.

Main Methods:

  • Overview of historical gene editing tools: meganucleases, zinc finger nucleases, and TALE nucleases.
  • Detailed examination of the CRISPR-Cas system, including its mechanism of DNA cleavage and cellular repair.
  • Summary of various CRISPR-Cas designs for diverse genomic modifications and gene expression modulation.

Main Results:

  • CRISPR-Cas is the most utilized gene editing method due to its ease of use, flexibility, and cost-effectiveness.
  • The system has been successfully applied to edit genomes in major food crops like maize, wheat, rice, and potatoes.
  • CRISPR-Cas enables modifications ranging from single-base changes to DNA fragment insertions and gene expression modulation (activation/repression).

Conclusions:

  • CRISPR-Cas technology has revolutionized precise genome modification in plants, offering significant potential for crop improvement.
  • The review highlights the versatility of CRISPR-Cas for various genetic manipulations and its impact on plant biotechnology.
  • Current challenges and prerequisites for effective CRISPR-Cas-mediated plant genome editing are also addressed.