Related Experiment Video
Updated: Dec 13, 2025

An Aptamer-based Sensor for Unchelated GadoliniumIII
Published on: January 9, 2017
Unusual absence of FRET in triazole bridged coumarin-hydroxyquinoline, an active sensor for Hg2+ detection
Surajit Mondal1, Niladri Patra1, Hari Pada Nayek1
1Department of Chemistry, Indian Institute of Technology (Indian School of Mines), Dhanbad, Jharkhand, India.
Abstract:
A triazole-bridged coumarin conjugated quinoline sensor has been 'click'-synthesized by Cu(i) catalyzed Huisgen cycloaddition, and it exhibited high selectivity for toxic Hg2+. Surprisingly, no evidence of energy transfer from the quinoline moiety to coumarin has been found, substantiated by time-resolved fluorescence study. The possible binding mode of this sensor to Hg2+ has been established via NMR study, steady-state and time-resolved fluorescence spectroscopy, which is further supported by TDDFT calculations. The sensor has been found to be cell membrane permeable and non-toxic, and hence is suitable for intracellular Hg2+ detection.
Related Concept Videos
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)
Colors and Magnetism
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human...
Valence Bond Theory
Aromatic Hydrocarbon Cations: Structural Overview
Removing one hydrogen from the intervening CH2 group...
2D NMR: Overview of Heteronuclear Correlation Techniques

