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Sortase mutants with improved protein thermostability and enzymatic activity obtained by consensus design

Magdalena Wójcik1, Susana Vázquez Torres1, Wim J Quax1

  • 1Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, A. Deusinglaan 1, 9713 Groningen, The Netherlands.

Protein Engineering, Design & Selection : PEDS
|July 30, 2020
PubMed
Summary

Researchers improved Staphylococcus aureus sortase A (SaSrtA) enzyme activity and stability. New SaSrtA variants are Ca2+-independent, enabling new biotechnology applications in low calcium environments.

Keywords:
Staphylococcus aureus sortase AConjugationconsensus designprotein engineeringprotein stability

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