Structural Characterization of an Exopolysaccharide Isolated from Enterococcus faecalis, and Study on its Antioxidant...

An exopolysaccharide (EPS-I) having the molecular weight ~ 2.6 × 10⁵ Da, was isolated from a Zinc resistant strain of Enterococcus faecalis from costal area. The exopolysaccharide consists of D-mannose, D-glucose, and L-fucose in molar ratio of 9:4:1. The monosaccharide units in the EPS-1 were determined through chemical (total acid hydrolysis and methylation analysis) and spectroscopic (FTIR and ¹H NMR experiment) analysis. The mannose-rich EPS-1 showed total antioxidant activity (1 mg mL^-1 of EPS-I as functional as approximately to 500 ± 5.2 µM of ascorbic acid) and Fe²⁺ metal ion chelation activity (EC₅₀ = 405.6 µg mL^-1) and hydroxyl radical scavenging activity (EC₅₀ = 219.5 µg mL^-1). The in vitro cytotoxicity experiment of EPS-I against cervical carcinoma cell line, HeLa cells showed strong cytotoxic effect (LC₅₀ = 267.3 µg mL^-1) and at that concentration, it found almost nontoxic against normal healthy cells (HEK-293).