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Long Noncoding RNA Expression in Adrenal Cortical Neoplasms.

Oyewale Shiyanbola1, Heather Hardin1, Rong Hu1

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Summary
This summary is machine-generated.

Long noncoding RNAs (lncRNAs) are key in tumorigenesis. This study found metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) expression is higher in adrenal cortical carcinomas (ACCs), aiding tumor classification.

Keywords:
Adrenal cortical carcinomaImmunohistochemistryIn situ hybridizationLong noncoding RNA

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Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
  • Distinguishing between adrenal cortical adenomas (ACAs) and carcinomas (ACCs) can be challenging, particularly with small biopsy samples.

Purpose of the Study:

  • To investigate the expression patterns of specific lncRNAs (MALAT1, PRINS, HAM1) in normal adrenal cortex, ACAs, and ACCs.
  • To assess the potential of these lncRNAs as biomarkers for classifying adrenal cortical tumors.

Main Methods:

  • In situ hybridization (ISH) and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) were employed.
  • A tissue microarray (TMA) was constructed using samples from normal adrenal cortex (n=23), ACAs (n=95), and ACCs (n=20).
  • ISH results were quantified using image analysis.

Main Results:

  • lncRNAs (MALAT1, PRINS, HAM1) were detected in all adrenal cortical tissues.
  • Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) showed significantly higher expression in ACCs compared to normal adrenal cortex and ACAs (p < 0.05).
  • Psoriasis susceptibility-related RNA gene induced by stress (PRINS) exhibited higher expression in normal adrenal cortex and ACAs than in ACCs.

Conclusions:

  • The lncRNAs MALAT1, PRINS, and HAM1 are expressed in normal and neoplastic adrenal tissues.
  • Elevated MALAT1 expression in ACCs suggests its potential role in adrenal cortical carcinoma development and classification.