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Updated: Dec 13, 2025
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Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
Published on: February 12, 2016
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Circulating Treg cells from patients with cerebral aneurysms displayed deficiency in ICOS expression and function
Ming-Guang Zhao1, Cheng Peng1, Li-Ming Li2
1Department of Neurosurgery, General Hospital of Northern Theater Command, Shenyang, China.
Clinical and Experimental Pharmacology & Physiology
|July 30, 2020
Summary
Inducible costimulator (ICOS) deficiency in regulatory T cells (Tregs) is linked to cerebral aneurysms. Restoring ICOS function may improve Treg stability and suppress inflammation in these conditions.
Area of Science:
- Immunology
- Vascular Biology
- Cellular Biology
Background:
- Inducible costimulator (ICOS) is crucial for T regulatory cell (Treg) differentiation and function.
- Cerebral aneurysms (CAs) are associated with inflammation and potential Treg dysfunction.
- FOXP3+ Treg cells play a critical role in immune regulation and tissue homeostasis.
Purpose of the Study:
- To investigate the expression and function of ICOS in Treg cells from patients with cerebral aneurysms.
- To determine the impact of ICOS signaling on Treg cell stability and suppressive capacity.
- To explore the potential therapeutic role of ICOS in CA pathogenesis.
Main Methods:
- Flow cytometry was used to analyze ICOS expression on circulating CD4+ CD25hi T cells.
- In vitro stimulation assays were performed using anti-ICOS, anti-CD3, and IL-2.
- Cytokine expression (IL-10, TGF-β, IL-17) and T cell proliferation assays were conducted.
Main Results:
- CA patients exhibited lower ICOS expression on circulating Tregs compared to healthy controls.
- Anti-ICOS costimulation enhanced FOXP3 expression and Treg suppressive function in healthy individuals.
- Tregs from CA patients showed reduced responsiveness to ICOS stimulation.
- ICOS signaling promoted IL-10 and TGF-β expression while suppressing IL-17.
Conclusions:
- Reduced ICOS expression and impaired responsiveness in Tregs may contribute to CA pathogenesis.
- ICOS signaling is vital for maintaining Treg stability and enhancing their immunosuppressive functions.
- Targeting ICOS may represent a potential therapeutic strategy for cerebral aneurysms.
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