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Analysis of the phase variation in lambda reduced immunity lysogens.

J Schneider, H B Strack

    Molecular & General Genetics : MGG
    |March 7, 1977
    PubMed
    Summary

    This study identifies single and tandem double lysogens in E. coli, revealing distinct immunity levels. Phage xis and host recA functions regulate transitions between these lysogenic states.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Some E. coli strains lysogenic for bacteriophage lambda exhibit reduced immunity.
    • Lysogenic E. coli can exist in distinct states with varying immunity levels.

    Purpose of the Study:

    • To characterize distinct lysogenic phases in E. coli with reduced immunity mutants of bacteriophage lambda.
    • To investigate the genetic regulation of transitions between lysogenic states.

    Main Methods:

    • DNA-DNA hybridization experiments were used to identify single and tandem double lysogens.
    • The roles of phage xis and host recA functions in lysogen transitions were examined.

    Main Results:

    • Two distinct phases, single and tandem double lysogens, were identified based on immunity levels and genetic markers.
    • The phage xis function is required for single to double lysogen transition.
    • The host recA function is required for double to single lysogen transition.
    • High-immunity 'rim' lysogens with multiple lambda genome copies were observed.

    Conclusions:

    • Different immunity levels in lysogenic E. coli reflect CI gene dosage effects.
    • The transition dynamics between lysogenic states are regulated by specific phage and host factors.
    • Unexplained high frequency of double lysogen formation from rim single lysogens was noted.

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