Quantifying the fractional concentrations and exchange rates of small-linewidth CEST agents using the QUCESOP method...

To develop a novel method for quantifying the fractional concentration (f_b ) and the exchange rate (k_b ) of a specific small-linewidth chemical exchange saturation transfer (CEST) solute in the presence of other unknown CEST solutes.

A simplified R_1ρ model was proposed assuming a small linewidth of the specific solute and a linear approximation of the other solutes' contribution to R_1ρ . Two modes of CEST data acquisition, using various saturation offsets and various saturation powers, were used. The f_b and k_b of the specific solute could be fitted using the proposed model. In MRI experiments, using either single-solute or multi-solute phantoms with various creatine concentrations and pHs, the f_b and k_b values of creatine were calculated for each phantom; the f_b and k_b values of phosphocreatine in rats' skeletal muscles were also evaluated.

The fitted f_b value of creatine from the phantoms were in excellent agreement. The fitted k_b value of creatine from the phantoms coincides with that from the literature, as do the f_b and k_b values of phosphocreatine in skeletal muscles.

The proposed approach enables us to quantify the f_b and k_b values of a specific small-linewidth solute in the presence of other unknown solutes.