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Published on: December 5, 2020
SYAUP-CN-26 applies its antifungal activity against Botrytis cinerea by disrupting mitochondrial structure and
Tingyue Feng1, Jialun Li1, Mingfan Sun1
1College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China.
Abstract:
This study investigated the effect of SYAUP-CN-26 on mitochondrial structure and function of Botrytis cinerea. The mitochondria, with the addition of SYAUP-CN-26 (EC50 [1.823 mg/L], EC90 [19.263 mg/L], and minimum inhibitory concentration [MIC] [79.754 mg/L]), emerged malformed shape, rough surface and unordered structure. As the concentration of SYAUP-CN-26 increases, the decrease in ATP content and the enhancement in the inhibition of mitochondrial respiratory chain complexes function confirmed that mitochondrial function was disrupted. And the respiratory superposing inhibition showed that SYAUP-CN-26 inhibited the tricarboxylic acid cycle (TCA) pathway of B. cinerea cells. Overall, these results indicated that SYAUP-CN-26 could inhibit mitochondrial structure and function to effect the growth of B. cinerea cells, and inhibition of mitochondrial respiratory chain complexes was a key factor for disruption of B. cinerea mitochondrial function and antifungal activity.
Insights
SYAUP-CN-26 disrupts the structure and function of Botrytis cinerea mitochondria. This antifungal compound inhibits mitochondrial respiration and the tricarboxylic acid cycle, impacting fungal growth.
Area of Science:
- Mycology
- Biochemistry
- Plant Pathology
Background:
- Botrytis cinerea is a significant fungal pathogen affecting various crops.
- Mitochondrial function is crucial for fungal growth and pathogenicity.
- Understanding antifungal mechanisms is vital for disease management.
Purpose of the Study:
- To investigate the effects of SYAUP-CN-26 on the mitochondria of Botrytis cinerea.
- To elucidate the specific mechanisms by which SYAUP-CN-26 exerts its antifungal activity.
- To determine the relationship between mitochondrial disruption and antifungal efficacy.
Main Methods:
- Exposure of Botrytis cinerea to varying concentrations of SYAUP-CN-26.
- Microscopic analysis of mitochondrial morphology.
- Measurement of ATP content.
- Assessment of mitochondrial respiratory chain complex activity.
- Analysis of tricarboxylic acid (TCA) cycle inhibition.
Main Results:
- SYAUP-CN-26 caused malformation and structural disorganization of Botrytis cinerea mitochondria.
- Increased SYAUP-CN-26 concentrations led to decreased ATP production and inhibited mitochondrial respiratory chain complexes.
- Respiratory superposing inhibition indicated disruption of the TCA pathway.
- Effective concentrations were determined: EC50 [1.823 mg/L], EC90 [19.263 mg/L], and MIC [79.754 mg/L].
Conclusions:
- SYAUP-CN-26 effectively inhibits the growth of Botrytis cinerea by targeting mitochondrial structure and function.
- Inhibition of mitochondrial respiratory chain complexes is a key mechanism underlying the antifungal activity of SYAUP-CN-26.
- Disruption of the TCA pathway contributes to the overall antifungal effect.
