Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

StableTi<sub>3</sub>C<sub>2</sub>T<sub>x</sub> MXene Ink Formulation and High-Resolution Aerosol Jet Printing for High-Performance MXene Supercapacitors.

Small methods·2025
Same author

Mitigating dithiothreitol interference to gold/thiol interface in electrochemical detection of cathepsin B activity toward multiplex protease analysis.

Biosensors & bioelectronics·2025
Same author

Investigation of HCAR2 antagonists as a potential strategy to modulate bovine leukocytes.

Journal of animal science and biotechnology·2024
Same author

Chemical acylation of pea protein isolate hydrolysate with fatty acid N-hydroxysuccinimide esters: Effect on structure and functional properties.

Food chemistry·2024
Same author

Enhanced Electron Transfer Rates by AC Voltammetry for Ferrocenes Attached to the End of Embedded Carbon Nanofiber Nanoelectrode Arrays.

Electroanalysis·2023
Same author

Synthesis of Chiral Tricyclic Pyrone Molecules via Palladium(0)-Catalyzed Displacement Reactions of Chiral Tricyclic Pyrone Acetate With Azide or Amine.

ChemistrySelect·2023
Same journal

AI-driven photophysics-aware design of fluorescent probes with applications in α-synuclein biosensing and inhibitor screening.

Biosensors & bioelectronics·2026
Same journal

Three-dimensional helical integration of high-density linear microelectrode arrays and their cross-tissue applications.

Biosensors & bioelectronics·2026
Same journal

Integration of electrochemical sensors in organ-on-a-chip microfluidic platforms: Advances and perspectives.

Biosensors & bioelectronics·2026
Same journal

DNN-PURE: A deep neural network approach to paper-based urea sensing.

Biosensors & bioelectronics·2026
Same journal

Rationally architected MOF-derived Co<sub>3</sub>O<sub>4</sub>@NiMn-LDH hollow heterostructure-based sensor array empowering sensitive detection and discrimination of neurological biomarkers.

Biosensors & bioelectronics·2026
Same journal

Four-in-one multifunctional CoCu-NC@AuPt nanozyme integrated M13 phage-displayed nanobody based multimodal lateral flow immunoassay for bovine lactoferrin detection.

Biosensors & bioelectronics·2026
See all related articles

Related Experiment Video

Updated: Dec 13, 2025

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays
10:44

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

Published on: August 26, 2013

14.4K

Simultaneous, multiplex quantification of protease activities using a gold microelectrode array.

Morgan J Anderson1, Yang Song2, Huafang Fan2

  • 1Department of Chemistry, Kansas State University, Manhattan, KS, 66506-0401, United States; Universities Space Research Association, NASA Ames Research Center, Moffett Field, CA, 94035, United States.

Biosensors & Bioelectronics
|July 31, 2020
PubMed
Summary
This summary is machine-generated.

This study presents a novel 3x3 gold microelectrode array for detecting cathepsin B activity. This electrochemical sensor platform enables rapid, multiplexed protease profiling for disease diagnostics, including cancer.

Keywords:
AC VoltammetryCathepsin BMicroelectrode arrayProtease activityProteolysis

More Related Videos

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
08:50

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Published on: October 10, 2013

12.0K
Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

1.9K

Related Experiment Videos

Last Updated: Dec 13, 2025

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays
10:44

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

Published on: August 26, 2013

14.4K
Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
08:50

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Published on: October 10, 2013

12.0K
Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

1.9K

Area of Science:

  • Electrochemistry
  • Biotechnology
  • Enzyme kinetics

Background:

  • Proteases are crucial enzymes in biological processes.
  • Accurate protease activity detection is vital for diagnosing and monitoring diseases like cancer.

Purpose of the Study:

  • To develop and characterize a 3x3 gold microelectrode array for multiplexed protease detection.
  • To demonstrate the platform's capability for rapid and accurate cathepsin B activity profiling.

Main Methods:

  • Fabrication of an individually addressable 3x3 gold microelectrode array.
  • Functionalization of microelectrodes with ferrocene-labeled peptide substrates.
  • Electrochemical detection using cyclic and AC voltammetry to monitor substrate cleavage.

Main Results:

  • Consistent electrochemical signals from individual microelectrodes.
  • Accurate measurement of cathepsin B proteolytic kinetics via AC voltammetry signal decay.
  • Demonstrated simultaneous detection of cathepsin B activity using three different peptide substrates.

Conclusions:

  • The developed microelectrode array offers a sensitive and multiplexed platform for protease activity profiling.
  • This technology holds significant potential for rapid disease diagnostics, particularly in oncology.
  • The improved fitting algorithm enhances the accuracy of protease activity determination.