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FnCas12a/crRNA assisted dumbbell-PCR detection of IsomiRs with terminal and inner sequence variants
Junyan Wang1, Tao Bing1, Nan Zhang1
1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS, Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China. bingtao@iccas.ac.cn sgdh@iccas.ac.cn and University of the Chinese Academy of Sciences, Beijing 100049, China.
Abstract:
Herein, we report a FnCas12a/crRNA assisted Dumbbell-PCR method for the detection of isomiRs with double specificity and magnification. The single nucleotide variant of isomiRs in terminals and/or inner sequence could be discriminated by this strategy. Using this method, let-7a isomiRs in MCF-7 and MCF-7R cell lines were analyzed.
