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Updated: Dec 13, 2025
Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Published on: February 1, 2014
A novel method for sensitive detection of Escherichia coli O157:H7 based on an aptamer and hybridization chain
Yuexin Liu1, Ping Chen, Shuai Yuan
1Jilin Agricultural University, College of Food Science and Engineering, Xincheng Street, Changchun, 130118, China. 1078663779@qq.com.
Abstract:
This work developed a novel assay for the detection of Escherichia coli O157:H7 (E. coli O157:H7), which is based on the specific recognition of an aptamer (H0) for E. coli O157:H7, the signal amplification of a hybridization chain reaction (HCR) and the adsorption and quenching effect of graphene oxide (GO). The feasibility of this work was verified through control experiments. The concentration of GO, the HCR time, the concentration of H0, and the concentration of H1/H2 were optimized. A series of E. coli O157:H7 dilutions were detected under the optimal conditions, and the detection range was 2.6 × 102 to 2.6 × 108 CFU mL-1. The linear equation is y = 591.1x- 943.9, R2 = 0.995, and LOD is 5 × 101 CFU mL-1. Milk samples were used for the spike recovery experiment, and the recovery rate was 92.6-103.6%. This method shortens the detection time greatly and provides a new strategy for food safety monitoring.
