Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.1K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.1K
Histone Modification02:32

Histone Modification

15.5K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
15.5K
Histone Modification02:32

Histone Modification

4.1K
4.1K
Heterochromatin02:38

Heterochromatin

17.4K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
17.4K
Heterochromatin02:38

Heterochromatin

4.4K
4.4K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.8K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The STA1-DOT2 interaction promotes nuclear speckle formation and splicing robustness in growth and heat stress responses.

The Plant cell·2026
Same author

A β-1,3-glucanase antagonizes a phase-separating WRKY repressor to confer saline-alkaline tolerance in rice.

The New phytologist·2026
Same author

Uncovering the role of ABI2 as a key regulator of flowering time in Arabidopsis.

Plant signaling & behavior·2026
Same author

Unveiling the epigenetic and stress-responsive function of histone H1 in plants.

Plant signaling & behavior·2026
Same author

Unlocking translational control of specialized metabolism in plants through 5'UTR structure.

Science advances·2026
Same author

A GPS2-like protein interacts with HOS15 and HDA6 to form a repressor complex that regulates ABA signaling and drought adaptation in Arabidopsis.

Plant communications·2026

Related Experiment Video

Updated: Dec 13, 2025

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.8K

The Histone-Modifying Complex PWR/HOS15/HD2C Epigenetically Regulates Cold Tolerance.

Chae Jin Lim1,2, Junghoon Park1,2, Mingzhe Shen3

  • 1Institute of Glocal Disease Control, Konkuk University, Seoul 05029, Republic of Korea.

Plant Physiology
|August 1, 2020
PubMed
Summary

The POWERDRESS protein is crucial for plant cold tolerance. It works with HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE15 and HISTONE DEACETYLASE2C to regulate cold-responsive genes and freezing survival.

More Related Videos

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
09:25

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

Published on: June 2, 2021

3.2K
Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae
11:06

Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae

Published on: December 29, 2017

13.1K

Related Experiment Videos

Last Updated: Dec 13, 2025

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.8K
Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
09:25

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

Published on: June 2, 2021

3.2K
Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae
11:06

Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae

Published on: December 29, 2017

13.1K

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Epigenetics

Background:

  • Environmental stress, particularly cold, significantly impacts plant growth and crop yields.
  • HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE15 (HOS15) is a key component of the CLR4 ubiquitin E3 ligase complex, regulating cold tolerance via epigenetic modifications.
  • HOS15 facilitates cold tolerance by degrading HISTONE DEACETYLASE2C (HD2C), altering chromatin structure from repressive to permissive.

Purpose of the Study:

  • To investigate the function of POWERDRESS (PWR), a HOS15-binding protein, in the plant cold stress response.
  • To elucidate the molecular mechanism by which PWR contributes to cold tolerance in Arabidopsis.

Main Methods:

  • Characterization of PWR loss-of-function mutants (pwr) in Arabidopsis thaliana.
  • Analysis of cold-regulated (COR) gene expression and freezing sensitivity in wild-type and pwr plants.
  • Co-immunoprecipitation assays to study protein-protein interactions between PWR, HOS15, and HD2C.
  • Chromatin immunoprecipitation (ChIP) to assess the association of HOS15 and HD2C with COR gene promoters.
  • Histone acetylation analysis to evaluate epigenetic modifications.

Main Results:

  • pwr mutants exhibit reduced expression of COR genes and increased sensitivity to freezing.
  • PWR interacts with HD2C via HOS15, and this interaction is essential for cold-induced HD2C degradation by HOS15.
  • The binding of HOS15 and HD2C to COR gene promoters is dependent on PWR.
  • Cold-induced histone H3 acetylation at COR gene promoters is significantly reduced in pwr mutants.
  • PWR modulates the binding of C-repeat element-binding factor transcription factors to COR gene promoters.

Conclusions:

  • The PWR-HOS15-HD2C complex is a critical regulator of COR gene expression and plant freezing tolerance.
  • PWR plays an essential role in the epigenetic reprogramming of chromatin structure in response to cold stress.
  • Understanding this pathway provides insights into enhancing crop resilience to cold environments.