Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

11.5K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.5K
Ribosome Profiling02:24

Ribosome Profiling

4.0K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of preeclampsia-associated immune-related risk loci in the Chinese population.

Taiwanese journal of obstetrics & gynecology·2026
Same author

Plant-Derived Thylakoids Potentiate Copper-Mediated Multimodal Cell Death via Hypoxia Alleviation for Synergistic Antitumor Therapy.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

PepAnno: A structure-aware deep learning framework for bioactive peptide prediction, structural visualization, and physicochemical profiling.

PLoS computational biology·2026
Same author

DDX24 exacerbates inflammation-induced immunosuppression in oral squamous cell carcinoma progression through IL-17 signaling pathway.

Archives of oral biology·2026
Same author

AWmeta Empowers Adaptively Weighted Transcriptomic Meta-Analysis.

Current issues in molecular biology·2026
Same author

Aging-associated decline of phosphatidylcholine synthesis is a malleable trigger of natural mitochondrial aging.

Nature communications·2026

Related Experiment Video

Updated: Dec 13, 2025

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
08:49

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

Published on: September 16, 2019

8.0K

Improving the diversity of captured full-length isoforms using a normalized single-molecule RNA-sequencing method.

Yueming Hu1, Xing-Sheng Shu1, Jiaxian Yu1

  • 1School of Basic Medicine, Shenzhen University Health Science Center, Shenzhen, 518060, China.

Communications Biology
|August 1, 2020
PubMed
Summary

A new cDNA normalization method significantly enhances the capture of full-length human gene isoforms using long-read RNA sequencing. This improved technique detects more lowly expressed isoforms and aids in identifying cancer-specific transcriptome signatures.

More Related Videos

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

7.8K
Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.2K

Related Experiment Videos

Last Updated: Dec 13, 2025

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
08:49

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

Published on: September 16, 2019

8.0K
A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

7.8K
Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.2K

Area of Science:

  • Molecular Biology
  • Genomics
  • Transcriptomics

Background:

  • Human genes generate diverse isoforms post-transcription, enabling varied functions.
  • Single-molecule RNA sequencing offers accurate isoform identification with long reads.
  • Current methods capture limited gene and isoform diversity.

Purpose of the Study:

  • To improve the capture efficiency of full-length, high-quality RNA sequencing isoforms.
  • To enhance the detection of lowly expressed and allele-specific transcripts.
  • To apply an improved protocol for profiling cancer transcriptomes.

Main Methods:

  • Implementing a cDNA normalization procedure prior to PacBio RS II sequencing library preparation.
  • Comparing normalized versus non-normalized capture procedures for isoform diversity.
  • Utilizing long-read RNA sequencing for transcriptome profiling of human gastric signet-ring cell carcinomas.

Main Results:

  • The cDNA normalization procedure increased the capture of full-length isoform species by 3.2-6.0 fold.
  • Lowly expressed, functionally significant isoforms were detected more effectively.
  • Normalized sequencing resolved a greater number of allele-specific haplotype transcripts.
  • New cancer-specific transcriptome signatures were identified in gastric carcinomas.

Conclusions:

  • cDNA normalization significantly boosts the yield of high-quality, full-length isoforms in long-read RNA sequencing.
  • This enhanced method improves the detection of rare and functionally important transcripts.
  • The improved protocol is valuable for comprehensive gene expression studies and cancer research.