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Updated: Jul 16, 2026

Measurement of Cytosolic Ca2+ in Isolated Contractile Lymphatics
Published on: December 8, 2011
Is an early calcium flux necessary to stimulate lymphocytes?
Abstract:
Concentrations of concanavalin A or the calcium ionophore A23187 that are optimal for the transformation of pig or mouse lymphocytes do not normally cause a measurable increase in calcium influx compared with unstimulated cells. If the cells are treated with the mitogens in conditions where a measurable increase in calcium influx occurs, no stimulation of the cells can occur while the flux is maintained. If an early influx of extracellular calcium is necessary for stimulation, then a much smaller increase in the total concentration of cellular calcium than reported previously is sufficient to allow the entry of lymphocytes into the cell cycle.
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