Large-Scale Expansion of Human Mesenchymal Stem Cells
Muhammad Najib Fathi Bin Hassan1, Muhammad Dain Yazid1, Mohd Heikal Mohd Yunus2
1Tissue Engineering Centre, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, 56000 Kuala Lumpur, Malaysia.
Stem Cells International
|August 1, 2020
Summary
Large-scale expansion of mesenchymal stem cells (MSCs) is crucial for cell therapy. Bioreactors and multilayered flasks offer higher expansion ratios while maintaining MSC quality and potency.
Area of Science:
- Regenerative Medicine
- Cell Biology
- Biotechnology
Background:
- Mesenchymal stem cells (MSCs) possess potent immunosuppressive properties, making them valuable for cell therapy.
- Clinical trials have explored MSCs for various conditions, including liver diseases, autoimmune disorders, and cardiovascular damage.
- Therapeutic applications necessitate large quantities of MSCs, ranging from millions to hundreds of millions per patient.
Purpose of the Study:
- To systematically review and discuss bioprocessing strategies for large-scale expansion of mesenchymal stem cells (MSCs).
- To evaluate the efficacy and impact of different expansion methods on MSC phenotype and potency.
Main Methods:
- A systematic literature search was conducted in Medline and Scopus databases.
- Twenty-six articles meeting inclusion criteria were reviewed, detailing MSC expansion from 7 sources.
- Four bioprocessing strategies were analyzed: bioreactor, spinner flask, roller bottle, and multilayered flask.
Main Results:
- Bioreactors, spinner flasks, and multilayered flasks were more frequently employed for MSC upscaling than roller bottles.
- Bioreactors and multilayered flasks generally yielded higher MSC expansion ratios.
- Expanded MSCs consistently maintained their phenotype and potency across all evaluated bioprocessing strategies.
Conclusions:
- Bioreactors, spinner flasks, roller bottles, and multilayered flasks are viable for large-scale MSC expansion.
- These methods can be utilized without compromising the critical quality attributes of mesenchymal stem cells.
- Effective large-scale MSC expansion strategies are essential for advancing cell-based therapies.
