Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

iPS Cell Differentiation01:22

iPS Cell Differentiation

3.0K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
3.0K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

5.1K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.1K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

26.8K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
26.8K
Stem Cell Culture01:17

Stem Cell Culture

5.9K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
5.9K
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

3.2K
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
3.2K
Embryonic Stem Cells00:58

Embryonic Stem Cells

31.6K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
31.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Applications of Stem Cell-Derived Islets and Emerging Experimental Platforms to Understand Diabetes.

Diabetes·2026
Same author

Modeling immune responses to autologous and allogeneic human stem cell-derived islet grafts in vivo.

JCI insight·2026
Same author

Wireless battery-free oxygenation devices enable extended immunosuppression-free islet transplantation in minimally invasive sites.

Device·2026
Same author

Optimizing post-transplantation detection of subcutaneously transplanted islets using dithizone staining.

BMC methods·2026
Same author

Divergent cell-type specific hypoxia responses in human stem cell-derived and primary islets.

Scientific reports·2026
Same author

A digital twin of pancreatic islet differentiation predicts cell fate.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Dec 13, 2025

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System
10:12

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

Published on: December 16, 2021

3.1K

Advances Toward Engineering Functionally Mature Human Pluripotent Stem Cell-Derived β Cells.

Leonardo Velazco-Cruz1, Madeleine M Goedegebuure1,2, Jeffrey R Millman1,2

  • 1Division of Endocrinology, Metabolism and Lipid Research, Washington University School of Medicine, St. Louis, MO, United States.

Frontiers in Bioengineering and Biotechnology
|August 1, 2020
PubMed
Summary

Human stem cell-derived beta (SC-β) cells show promise for diabetes treatment. Recent advances focus on improving SC-β cell differentiation and function for effective glucose control and potential transplantation therapies.

Keywords:
diabetesdifferentiationpluripotentstem cellstransplantation

More Related Videos

Author Spotlight: Advancements and Challenges in β-Cells Differentiation from Pluripotent Stem Cells
06:33

Author Spotlight: Advancements and Challenges in β-Cells Differentiation from Pluripotent Stem Cells

Published on: February 2, 2024

2.4K
Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters
08:41

Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters

Published on: June 23, 2023

3.9K

Related Experiment Videos

Last Updated: Dec 13, 2025

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System
10:12

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

Published on: December 16, 2021

3.1K
Author Spotlight: Advancements and Challenges in β-Cells Differentiation from Pluripotent Stem Cells
06:33

Author Spotlight: Advancements and Challenges in β-Cells Differentiation from Pluripotent Stem Cells

Published on: February 2, 2024

2.4K
Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters
08:41

Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters

Published on: June 23, 2023

3.9K

Area of Science:

  • Biomedical Engineering
  • Stem Cell Biology
  • Endocrinology

Background:

  • Human stem cell-derived beta (SC-β) cells offer potential for diabetes treatment, including disease modeling, drug screening, and cellular therapy.
  • SC-β cells represent an early clinical translation of differentiated human pluripotent stem cells (hPSC).
  • Initial 2014 reports demonstrated in vitro glucose-responsive SC-β cells, but functional maturation remained limited.

Purpose of the Study:

  • To review recent advances in engineering SC-β cells.
  • To understand and improve SC-β cell differentiation and functional maturation.
  • To highlight strategies achieving dynamic glucose-responsive insulin secretion for potential transplantation.

Main Methods:

  • Review of recent scientific literature on SC-β cell engineering.
  • Analysis of novel differentiation strategies for hPSC-derived β cells.
  • Evaluation of functional maturation and glucose responsiveness in engineered SC-β cells.

Main Results:

  • Significant progress has been made in improving SC-β cell differentiation and functional maturation.
  • New strategies enable dynamic, glucose-responsive insulin secretion.
  • Transplantation studies in diabetic mice show rapid correction to normoglycemia.

Conclusions:

  • Engineered SC-β cells are advancing towards clinical applications for diabetes.
  • Improved differentiation and maturation are key to achieving therapeutic efficacy.
  • SC-β cell therapy holds significant promise for revolutionizing diabetes management.