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Optimization of ligation reaction conditions in gene synthesis
N Y Theriault1, J B Carter, S P Pulaski
1Biopolymer Chemistry, Upjohn Company, Kalamazoo, MI 49001.
Biotechniques
|May 1, 1988
Abstract:
Several phosphorylation, annealing and ligation reaction conditions have been investigated for the enzymatic assembly of genes of interest. The use of longer oligodeoxyribonucleotides (40-60 bases long) also improved the enzymatic reaction. Furthermore, the use of longer oligonucleotides and the elimination of long stretches of G's or C's lowered the mutation rate.