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Related Experiment Video

Updated: Dec 13, 2025

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Ceftriaxone pharmacokinetics by a sensitive and simple LC-MS/MS method: Development and application.

L Herrera-Hidalgo1, M V Gil-Navarro1, S Dilly Penchala2

  • 1Unidad de Gestión Clínica de Farmacia, Hospital Universitario Virgen del Rocío/CSIC/Instituto de Biomedicina de Sevilla (IBiS), Seville, Spain.

Journal of Pharmaceutical and Biomedical Analysis
|August 1, 2020
PubMed
Summary
This summary is machine-generated.

A new, rapid bioanalytical method using LC-MS/MS accurately quantifies ceftriaxone in human plasma. This validated assay supports therapeutic drug monitoring and research for optimized patient dosing.

Keywords:
CeftriaxoneDevelopmentLC–MS/MSPharmacokineticsValidation

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Area of Science:

  • Pharmacology
  • Analytical Chemistry
  • Clinical Diagnostics

Background:

  • Ceftriaxone, a vital third-generation cephalosporin, is crucial for treating severe infections like meningitis.
  • Evolving clinical practices necessitate precise ceftriaxone quantification due to high-dose regimens and individualized treatment approaches.

Purpose of the Study:

  • To develop and validate a rapid, sensitive bioanalytical method for quantifying ceftriaxone in human plasma.
  • To establish a reliable assay for therapeutic drug monitoring and research applications.

Main Methods:

  • High-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was employed.
  • Sample preparation involved protein precipitation with acetonitrile; chromatographic separation utilized a Phenomenex Luna C18 column.
  • Quantification was performed using positive electrospray ionization (ESI) within a 5-minute runtime.

Main Results:

  • The method demonstrated high sensitivity and robustness, validated over a concentration range of 3-1000 μg/mL.
  • Accuracy and precision were consistently below 15%, meeting U.S. FDA regulatory standards.
  • The assay provides a fast and reliable means for measuring ceftriaxone levels in plasma.

Conclusions:

  • A sensitive, robust, and rapid LC-MS/MS method for ceftriaxone quantification in human plasma has been successfully developed and validated.
  • This assay is suitable for research and clinical therapeutic drug monitoring, aiding in optimized ceftriaxone dosing.
  • The validated method addresses the growing need for precise drug level measurements in evolving clinical scenarios.