Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluating the applicability of computational super-resolution techniques for harmonic generation microscopy.

Scientific reports·2026
Same author

Evaluation of a time-resolved singlet oxygen detection system for in vivo photodynamic therapy.

Biomedical optics express·2026
Same author

Development of solid-state fluorescence lifetime standards for clinical applications using dyed epoxy resins.

Journal of biomedical optics·2026
Same author

Characterization of inner ear granular bodies by polarization-resolved second harmonic generation microscopy.

Biomedical optics express·2026
Same author

A Membrane Fluidization Strategy Significantly Boosts Photodynamic Therapy and Antitumor Immunity of Porphyrin-Lipid Nanoparticles.

ACS nano medicine·2026
Same author

In Vivo Assessment of Benzoporphyrin Uptake and Singlet Oxygen Generation in Mice for Photodynamic Therapy Monitoring.

ACS photonics·2026
Same journal

Changes in Three-Dimensional Intrahepatic Biliary Structures in Patients With Hepatobiliary Diseases Visualized Using Tissue-Clearing Methods.

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Genome-wide SNP-based Profiling of Loss of Heterozygosity Reveals Distinct Molecular Subgroup-specific Patterns in Gastrointestinal Stromal Tumors (GIST).

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

AI-Assisted HER2 Scoring in Breast Cancer: Diagnostic Agreement and Understanding Discordance.

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Corrigendum to "POU2F3 in Small Cell Lung Cancer (SCLC): Diagnostic Utility in Neuroendocrine-Low/Negative SCLC and Discrimination From Other Thoracic Malignancies and Other Small Blue Round Cell Tumors" [Laboratory Investigation 2026;106(6):106124].

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Assessing the Effects of a 3D Pathology Tissue-Processing Workflow on Downstream Molecular Analyses.

Laboratory investigation; a journal of technical methods and pathology·2026
Same journal

Transcription Factor Ets-1 is a Central Regulator of Redox Balance and Liver Regeneration Through EGF and TGF-β1 Signaling.

Laboratory investigation; a journal of technical methods and pathology·2026
See all related articles

Related Experiment Video

Updated: Dec 13, 2025

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
05:54

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

1.6K

Characterization of pathological thyroid tissue using polarization-sensitive second harmonic generation microscopy.

Danielle Tokarz1, Richard Cisek2, Ariana Joseph2

  • 1Department of Chemistry, Saint Mary's University, Halifax, NS, Canada. danielle.tokarz@smu.ca.

Laboratory Investigation; a Journal of Technical Methods and Pathology
|August 2, 2020
PubMed
Summary
This summary is machine-generated.

Polarization-sensitive second harmonic generation microscopy reveals altered collagen structures in diseased thyroid tissues. This technique offers a new way to distinguish between normal and cancerous thyroid using collagen

More Related Videos

Author Spotlight: Integrating Ultrasound Imaging with Biochemical Markers for Thyroid Disease Diagnosis
05:41

Author Spotlight: Integrating Ultrasound Imaging with Biochemical Markers for Thyroid Disease Diagnosis

Published on: February 9, 2024

952
An Ex vivo Culture System to Study Thyroid Development
08:33

An Ex vivo Culture System to Study Thyroid Development

Published on: June 6, 2014

12.0K

Related Experiment Videos

Last Updated: Dec 13, 2025

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
05:54

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

1.6K
Author Spotlight: Integrating Ultrasound Imaging with Biochemical Markers for Thyroid Disease Diagnosis
05:41

Author Spotlight: Integrating Ultrasound Imaging with Biochemical Markers for Thyroid Disease Diagnosis

Published on: February 9, 2024

952
An Ex vivo Culture System to Study Thyroid Development
08:33

An Ex vivo Culture System to Study Thyroid Development

Published on: June 6, 2014

12.0K

Area of Science:

  • Biophysics
  • Medical Imaging
  • Materials Science

Background:

  • Polarization-sensitive second harmonic generation (SHG) microscopy analyzes molecular structures like collagen.
  • Collagen ultrastructure changes are linked to various diseases.

Purpose of the Study:

  • To investigate collagen ultrastructural changes in normal versus diseased human thyroid tissues using SHG microscopy.
  • To explore SHG microscopy as a diagnostic tool for thyroid pathologies.

Main Methods:

  • Applied polarization-sensitive SHG microscopy to ex vivo human thyroid tissue slides.
  • Quantified second-order nonlinear optical susceptibility tensor component ratios (χ(2)zzz'/χ(2)zxx' and χ(2)xyz'/χ(2)zxx') and degree of linear polarization (DOLP).

Main Results:

  • Diseased thyroid tissues showed a significant increase in χ(2)zzz'/χ(2)zxx' (structural parameter) and a decrease in DOLP (chirality measure) compared to normal thyroid, except for insular carcinoma.
  • These changes indicate increased ultrastructural disorder in collagen within diseased tissues.

Conclusions:

  • SHG microscopy can detect significant alterations in collagen ultrastructure in diseased thyroid tissues.
  • This method provides a novel, complementary approach to histopathology for discriminating normal from diseased thyroid conditions.