Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LRRK2 Mutation Alters Dopamine D2 Receptor Localization in Induced Pluripotent Stem Cells-Derived Astrocytes From Parkinson's Disease Patients: Implications for Neuronal Damage.

Journal of neurochemistry·2026
Same author

Chromatin-associated intronic RNAs from long genes form introsomes that shape nuclear architecture in neuronal cells.

bioRxiv : the preprint server for biology·2026
Same author

Acquired genetic and cell-state changes in IDH-mutant glioma progression.

Nature·2026
Same author

ZFHX4 is necessary for dopaminergic neuron differentiation and controls cell cycle by regulating LIN28A.

Stem cell reports·2026
Same author

SARS-CoV-2 Infection Induces Dopaminergic Neuronal Loss in Midbrain Organoids.

Journal of neurochemistry·2026
Same author

Networked SIRS model with Kalman filter state estimation for epidemic monitoring in Europe.

Communications medicine·2026

Related Experiment Video

Updated: Dec 13, 2025

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies
05:45

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies

Published on: March 29, 2024

3.1K

Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids.

Lisa M Smits1, Stefano Magni1, Kaoru Kinugawa2

  • 1Luxembourg Centre for Systems Biomedicine (LCSB), Developmental and Cellular Biology, University of Luxembourg, Belvaux, Luxembourg.

Cell and Tissue Research
|August 2, 2020
PubMed
Summary
This summary is machine-generated.

Human midbrain organoids (hMOs) contain diverse neuronal subtypes, including dopaminergic, GABAergic, glutamatergic, and serotonergic neurons. These organoids exhibit functional properties, making them valuable for disease modeling and drug discovery.

Keywords:
Electrophysiological activityMidbrain organoidsNeural stem cellsNeuronal subtypesSingle-cell RNA sequencing

More Related Videos

Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases
09:36

Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases

Published on: June 28, 2019

10.4K
Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function
07:13

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function

Published on: November 29, 2024

1.7K

Related Experiment Videos

Last Updated: Dec 13, 2025

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies
05:45

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies

Published on: March 29, 2024

3.1K
Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases
09:36

Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases

Published on: June 28, 2019

10.4K
Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function
07:13

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function

Published on: November 29, 2024

1.7K

Area of Science:

  • Neuroscience
  • Stem Cell Biology
  • Organoid Technology

Background:

  • Human stem cell-derived organoids model organ structure and function in vitro.
  • Human midbrain organoids (hMOs) are known to contain dopaminergic neurons.
  • The human midbrain comprises multiple interacting neuronal cell types beyond dopaminergic neurons.

Purpose of the Study:

  • To analyze cell heterogeneity in hMOs at high resolution.
  • To investigate the functional properties of neuronal subtypes within hMOs.
  • To assess the utility of hMOs for disease modeling and drug discovery.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq) for high-resolution cell analysis.
  • Imaging techniques to visualize cellular organization.
  • Electrophysiology to assess neuronal functional properties.

Main Results:

  • hMOs exhibit significant neuronal cell heterogeneity.
  • Demonstrated spontaneous electrophysiological activity across multiple neuronal subtypes.
  • Identified dopaminergic, GABAergic, glutamatergic, and serotonergic neurons within hMOs.

Conclusions:

  • hMOs recapitulate key in vivo features of the human midbrain.
  • hMOs possess essential neuronal functional properties.
  • hMOs are a powerful tool for in vitro disease phenotyping and drug discovery.