Related Experiment Video
Updated: Aug 1, 2026
15:52
Postproduction Processing of Electrospun Fibres for Tissue Engineering
Published on: August 9, 2012
Electrospun Solid Formulation of Anaerobic Gut Microbiome Bacteria
Panna Vass1, Eszter Pantea1, András Domokos1
1Department of Organic Chemistry and Technology, Budapest University of Technology and Economics (BME), Műegyetem rakpart 3, Budapest, H-1111, Hungary.
AAPS Pharmscitech
|August 2, 2020
Summary
Clostridium butyricum, an anti-inflammatory gut bacterium, was successfully incorporated into water-soluble cyclodextrin fibers using high-speed electrospinning. These bacteria remained viable for 12 months, enabling oral tablet formulation.
Area of Science:
- Microbiology
- Biotechnology
- Materials Science
Background:
- Gut microbiome modulation is crucial for health.
- Clostridium butyricum produces anti-inflammatory molecules.
- Developing stable delivery systems for probiotics is challenging.
Purpose of the Study:
- To develop a novel delivery system for Clostridium butyricum.
- To assess the long-term viability of bacteria within the delivery system.
- To enable oral administration of the probiotic bacteria.
Main Methods:
- High-speed electrospinning of polymer-free hydroxypropyl-β-cyclodextrin (HP-β-CD) fibers.
- Incorporation of Clostridium butyricum into HP-β-CD fibers.
- Long-term stability testing under various storage conditions (temperature, oxygen).
- Fabrication of oral tablets via milling and direct compression.
Main Results:
- Viable Clostridium butyricum was successfully encapsulated in HP-β-CD fibers.
- Bacterial viability was maintained for 12 months at room temperature, even with oxygen exposure.
- Milling and direct compression for tableting did not significantly impact bacterial viability.
Conclusions:
- High-speed electrospinning is a scalable nanotechnology for probiotic delivery.
- HP-β-CD fibers provide a stable matrix for anaerobic gut bacteria.
- The developed system allows for convenient oral administration of viable Clostridium butyricum.
