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Improved detection methods significantly increase the detection window for EPO microdoses.

Laurent Martin¹, Jean-Antoine Martin¹, David Collot¹

¹Analysis Department - Agence Française de Lutte contre le Dopage (AFLD), Châtenay-Malabry, France.

Drug Testing and Analysis

|August 2, 2020

Summary

This summary is machine-generated.

Recombinant erythropoietin (rEPO) microdoses were detectable in blood and urine for up to 72 hours post-administration. Urine analysis using SDS-PAGE offered the most sensitive detection method for identifying rEPO doping.

Keywords:

doping detection erythropoietin microdoses serum urine

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Area of Science:

Sports Science
Biochemistry
Anti-doping Research

Background:

Recombinant erythropoietin (rEPO) is a performance-enhancing drug used in doping.
Detecting microdoses of rEPO presents challenges in anti-doping efforts.
Growth hormone (GH) co-administration is a potential doping strategy.

Purpose of the Study:

To evaluate the detection capabilities of rEPO in biological samples during and after microdose administration.
To assess the impact of GH co-administration on rEPO detection.
To refine anti-doping techniques for rEPO identification.

Main Methods:

Administration of rEPO microdoses, alone or with GH microdoses, to healthy male subjects.
Analysis of blood and urine samples using improved iso-electric focusing (IEF), SDS-PAGE, and SAR-PAGE methods.
Immuno-extraction and elution buffer modifications to enhance EPO detection in blood and urine.

Main Results:

rEPO microdoses were detectable in blood and urine between 24 and 72 hours post-administration.
Urine analysis with SDS-PAGE demonstrated the highest sensitivity for prolonged rEPO detection (91% at 72 hours).
GH co-administration did not affect rEPO elimination or detection.

Conclusions:

Improved anti-doping methods enhance the detection of rEPO microdoses.
Urine SDS-PAGE is a highly sensitive method for detecting rEPO doping.
rEPO microdosing is detectable for several days, aiding anti-doping efforts.