Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

510
Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
510
Protein-protein Interfaces02:04

Protein-protein Interfaces

14.3K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.3K
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

336
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
336
Mechanism of Conjugation01:19

Mechanism of Conjugation

574
Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...
574
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.8K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.8K
Fimbriae, Pili, and Axial Filaments01:28

Fimbriae, Pili, and Axial Filaments

1.2K
Fimbriae and pili are specialized bacterial surface structures that play pivotal roles in adhesion, genetic exchange, and motility. Composed primarily of pilin protein, these hairlike appendages are crucial for bacterial survival and pathogenicity in various environments.Fimbriae: Adhesion and PathogenicityFimbriae are fine, filamentous structures measuring 2–10 nanometers in diameter and are densely distributed on the bacterial cell surface. They facilitate bacterial adhesion to abiotic...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimising DNA origami assembly by reducing off-target interactions.

Nature communications·2026
Same author

SypC, a symbiont outer membrane vesicle protein, impacts the development of the squid-vibrio partnership.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

MtrR Regulates a Major Lytic Transglycosylase (<i>ltgA</i>) Responsible for Peptidoglycan-Derived Cytotoxin Release and Autolysis in <i>Neisseria gonorrhoeae</i>.

Microorganisms·2026
Same author

Genetic transformation of <i>Gardnerella</i> species and characterization of vaginolysin and sialidase mutants.

Infection and immunity·2025
Same author

Genetic transformation of <i>Gardnerella</i> species and characterization of vaginolysin and sialidase mutants.

bioRxiv : the preprint server for biology·2025
Same author

Context-Aware Biosensor Design Through Biology-Guided Machine Learning and Dynamical Modeling.

ACS synthetic biology·2025

Related Experiment Video

Updated: Dec 13, 2025

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
10:41

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Published on: January 4, 2017

14.2K

Protein interactions within and between two F-type type IV secretion systems.

Birgit Koch1, Melanie M Callaghan2, Jonathan Tellechea-Luzardo1

  • 1Interdisciplinary Computing and Complex BioSystems (ICOS), School of Computing Science, Newcastle University, Newcastle upon Tyne, UK.

Molecular Microbiology
|August 2, 2020
PubMed
Summary

Neisseria gonorrhoeae type IV secretion systems (T4SSs) share interactions with F-type systems. This study identified unique protein interactions in the N. gonorrhoeae T4SS, revealing features of its extracellular DNA secretion.

Keywords:
E. coliN. gonorrhoeaeF-plasmidprotein-protein interactionstwo-hybridtype IV secretion systems

More Related Videos

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates
07:43

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Published on: July 22, 2019

8.5K
Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
11:33

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking

Published on: December 17, 2013

6.4K

Related Experiment Videos

Last Updated: Dec 13, 2025

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
10:41

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Published on: January 4, 2017

14.2K
Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates
07:43

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Published on: July 22, 2019

8.5K
Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
11:33

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking

Published on: December 17, 2013

6.4K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Genetics

Background:

  • Bacterial type IV secretion systems (T4SSs) are crucial for horizontal gene transfer, including conjugation.
  • The Neisseria gonorrhoeae T4SS exhibits unique extracellular DNA secretion capabilities, distinguishing it from typical conjugative systems.
  • Homology suggests the N. gonorrhoeae T4SS can be classified with F-type conjugative T4SSs.

Purpose of the Study:

  • To investigate protein-protein interactions among 17 proteins involved in DNA secretion by the N. gonorrhoeae T4SS.
  • To compare these interactions with those of F-plasmid T4SS proteins to identify conserved and unique functional features.
  • To elucidate the molecular basis for the N. gonorrhoeae T4SS's distinctive extracellular DNA secretion.

Main Methods:

  • Utilized the Bacterial Two-Hybrid system with a Transmembrane (BACTH-TM) domain assay to study periplasmic protein interactions.
  • Employed cross-system comparisons by swapping interaction partners between N. gonorrhoeae and F-plasmid T4SSs.
  • Confirmed specific outer membrane protein interactions using co-purification techniques.

Main Results:

  • Identified known and novel protein interactions within both the N. gonorrhoeae and F-type T4SSs.
  • Demonstrated novel interactions between conserved outer membrane core proteins and F-type-specific proteins.
  • Confirmed that the F-type-specific protein TraHN localizes to the outer membrane, dependent on TraGN.

Conclusions:

  • The study reveals conserved and unique protein interaction networks within bacterial type IV secretion systems.
  • Specific protein interactions, particularly involving outer membrane components like TraHN and TraGN, are critical for the N. gonorrhoeae T4SS function.
  • Understanding these interactions provides insights into the mechanisms of extracellular DNA secretion in bacteria.