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Updated: Dec 13, 2025
Light-Controlled Fermentations for Microbial Chemical and Protein Production
Published on: March 22, 2022
Parametric optimization and kinetic study of l-lactic acid production by homologous batch fermentation of
Jianfei Wang1, Jiaqi Huang1,2, Shaoming Jiang1
1Department of Paper and Bioprocess Engineering, SUNY College of Environmental Science and Forestry, Syracuse, USA.
Abstract:
Parametric optimization always plays important roles in bioengineering systems to obtain a high product yield under the proper conditions. The parametric conditions of lactic acid production by homologous batch fermentation of Lactobacillus pentosus cells was optimized by the Box-Behnken design. The highest l-lactic acid yield was obtained as 0.836 ± 0.003 g/g glucose with the productivity of 0.906 ± 0.003 g/(L × H) under the optimum conditions of 34.7 °C, pH 6.2, 148 rpm agitation speed, and 9.3 g/L nitrogen source concentration determined by quadratic response surface with high accuracy. The adequate kinetic models of cell growth rate, lactic production rate, and glucose consumption rate were also established to describe the fermentation behavior of L. pentosus cells with the correlation coefficients of 09985, 0.9990, and 0.9989, respectively.
