A bisphenol based fluorescence chemosensor for the selective detection of Zn²⁺ and PPi ions and its bioluminescence...

A bisphenol based fluorescence "turn-on" chemosensor 4,4'-(propane-2,2-diyl)bis(2-((E)-(2-(benzo[d]thiazol-2-yl)hydrazineyldene)methyl)phenol) (BHMP) has been synthesized and its sensing behavior was tested towards various ionic species. The chemo-sensing behavior of BHMP has been established through absorption, fluorescence, NMR, and mass spectroscopic techniques. The probe BHMP selectively detects zinc ions over other metal ions and the resulting BHMP + Zn²⁺ ensemble serves as a secondary probe for the detection of pyrophosphate (PPi) anion specifically over other anions. The spectroscopic studies reveal the fluorescence enhancement of BHMP in association with Zn²⁺ ions was quenched in the presence of pyrophosphate (PPi) anions. A probable mechanism of this selective sensing behavior was described on the basis of "OFF-ON-OFF" strategy for detection of both cations and anions. Moreover, the biological applicability of the chemosensor BHMP was examined via cell imaging studies.