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Canine atopic dermatitis: Role of luteolin as new natural treatment
Enrico Gugliandolo1, Ernesto Palma2, Marika Cordaro1
1Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.
Background:
Luteolin has been demonstrated to possess numerous biological effects. However, the effect of luteolin on LPS (Lipopolysaccharides) stimulation in CPEK cells has not been investigated.
Hypothesis/Objectives:
An in vitro model of atopic canine dermatitis was used to identify the antioxidant effect of luteolin as a new treatment that is capable of improving the conditions of veterinary patients.
Methods:
CPEK cells were treated with or without luteolin in the presence or absence of LPS. A cell viability assay was performed to test luteolin toxicity and the protective effect of luteolin after LPS stimulation. Additionally, enzyme-linked immunosorbent assay (ELISA) kits were used to detect the levels of IL-33, IL-1β, IL-6, and IL-8.
Results:
Luteolin was capable to significantly decrease levels expression of IL-33, IL 1β, IL-6, and IL-8.
Conclusions And Clinical Importance:
Luteolin could be a new pharmacological treatment for canine atopic dermatitis.
