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Updated: Dec 13, 2025

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling
Published on: June 23, 2022
Early experience with universal preprocedural testing for SARS-CoV-2 in a relatively low-prevalence area
Sarah S Lewis1, Becky A Smith1, Ibukunoluwa C Akinboyo2
1Division of Infectious Diseases, Department of Medicine, Duke University, Durham, North Carolina.
Universal severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing for surgical patients identified a low rate of asymptomatic infections. This strategy successfully conserved personal protective equipment (PPE) and prevented healthcare worker exposure to coronavirus disease 2019 (COVID-19).
Area of Science:
- Infectious Diseases
- Public Health
- Surgical Safety
Background:
- The COVID-19 pandemic necessitated novel strategies for patient management and healthcare worker protection.
- Conserving personal protective equipment (PPE) became critical during surges of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections.
- Understanding the prevalence of asymptomatic SARS-CoV-2 in surgical populations is vital for infection control.
Purpose of the Study:
- To evaluate the implementation of universal SARS-CoV-2 testing in patients undergoing surgery.
- To assess the rate of asymptomatic SARS-CoV-2 infections within this patient cohort.
- To determine the effectiveness of universal testing in conserving PPE and preventing healthcare team exposure.
Main Methods:
- Implemented universal SARS-CoV-2 testing for all patients scheduled for surgical procedures.
- Monitored infection rates and PPE utilization.
- Tracked instances of potential healthcare worker exposure to SARS-CoV-2.
Main Results:
- The prevalence of asymptomatic SARS-CoV-2 infection among surgical patients was less than 0.5%.
- The testing protocol, while resource-intensive, successfully prevented exposures for healthcare team members.
- Findings suggest the efficacy of early local public health interventions in controlling community transmission.
Conclusions:
- Universal SARS-CoV-2 testing in surgical settings is a feasible strategy for infection control.
- The low rate of asymptomatic cases indicates successful public health measures in the studied region.
- This approach enhances surgical safety by protecting healthcare workers from potential coronavirus disease 2019 exposure.
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